Product Details
Catalog Number: 2-1240-001
Applications: Protein Purification
Storage: 2 - 8 °C
Shipping: Room Temperature
Downloads: Datasheet (pdf)
Downloads: MSDS (pdf)
Product Sizes
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As of 10/31/2024, Strep-Tactin® Sepharose® products have been discontinued. The replacement products are Strep-Tactin® 4Flow® High Capacity Products.

Replacement:

Advantages of Strep-Tactin® 4Flow® High Capacity vs. Strep-Tactin® Superflow®:

 Matrix

Strep-Tactin® Superflow®

Strep-Tactin® 4Flow®

Bead structure

6% agarose, crosslinked

4% agarose, highly crosslinked

Bead size

60-160 um

50-150 um, spherical

Exclusion limit

6 x 10^6 Da

3 x 10^7 Da

Recommended flow rate

0.5-1 ml/min

0.5-1 ml/min

pH range for protein binding

7-8

7-8

Max pressue

9.6 bar

3.5 bar

 Eluent

desthiobiotin

desthiobiotin

Regeneration buffer

Buffer R

100 mM NaOH

Strep-Tactin® Superflow® high capacity cartridge for one-step purification of recombinant Strep-tag® proteins with HPLC or FPLC.

The cartridges are primarily designed for use with chromatography workstations with 10-32 connections (please note that you do not need adapters for HPLC and Äkta). They can, however, also be operated with other workstations, with syringes or with peristaltic pumps by use of common adapters for cartridges. Since column bodies are pressure resistant ) for up to 3 bar, the cartridges can be used with a flow restrictor. Cartridges can be connected in series to enlarge capacity.

Dynamic binding capacity: 7mg protein/ml resin, determined with mCherry-Twin-Strep-tag® (28kDa)

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We are building our company one satisfied customer at a time. If you have any questions or concerns, please contact us.

Neuromics
5325 West 74th St., Suite 8
Edina, MN 55439

Toll free: 866-350-1500
Int’l phone: 952-374-6161
Fax: 612-677-3976