Protocols for Antibodies

• Fluorescence Immunohistochemistry
• Immunohistochemistry
  Sample IHC Protocol

  Sample IHC Protocol Using Frozen Tissue

  Sample IHC Protocol Using Paraffin Sections

  Immunostaining of Cells in Tissue Culture

• Western Blot
  Western Blot Protocol Designed for Cell Lysates

  Western Blot Protocol Including Electrophoresis Buffer

Protocols for Human Cells/Tissue

• Endothelial Cells
  Protocol for Frozen Endothelial Cells

  Protocol for Endothelial Cells in T25 Flask

  General Tips

• Hepatocytes
  Protocol for Hepatocytes

• NeuroProgenitors
  NeuroProgenitors

• Pericytes
  Protocol for Frozen Pericytes

  Protocol for Pericytes in T25 Flask

Protocols for Cell Based Assays

• ELISA
  Sample ELISA Capture Protocol

  Sample Sandwich ELISA Protocol

Protocols for Gene Tools

• i-Fect Transcription Kit
  i-Fect Transfection Kit Instruction Manual

  i-Fect Transfection Kit Supplement to Instruction Manual

• Sleeping Beauty Transposon
  Sleeping Beauty Transposon System User Manual