Strep-Tactin® 4Flow® High Capacity FPLC Column, 1 ml

Product Details

Catalog Number: 2-1257-001

Applications: Protein Purification, FPLC

Storage: 2-8°C

Shipping: Room Temperature

Product Sizes

Size	List Price	Price	Cart
1 ml		$166.00	Add to Cart
10 x 1 ml		$1,580.00	Add to Cart

On 10/31/2024, Strep-Tactin® Sepharose® and Strep-Tactin® Superflow® products will be discontinued and replaced with the new and improved Strep-Tactin® 4Flow® High Capacity Products. Learn more below.

Strep-Tactin® 4Flow® high capacity FPLC columns are ready-to-use columns for affinity chromatography. They are suitable for automated protein purification with HPLC/FPLC devices such as Äkta® systems (Cytiva). The FPLC columns contain a cross-linked agarose coupled to the ligand Strep -Tactin®, a streptavidin variant that binds to Strep-tag®II or Twin-Strep-tag® fusion proteins. This protein purification system is characterized by a high target purity of >95% and a stable performance even after several regeneration cycles. These columns are the perfect alternative for proteins that are difficult to elute from Strep-Tactin®XT 4Flow® and Strep-Tactin®XT 4Flow® high capacity FPLC columns.

Replacing:

Name	Catalog Number
Strep-Tactin® Superflow® High Capacity FPLC Column	2-1239-001

Advantages of Strep-Tactin® 4Flow® High Capacity vs. Strep-Tactin® Sepharose® and Strep-Tactin® Superflow®:

Matrix	Strep-Tactin® Sepharose®	Strep-Tactin® Superflow®	Strep-Tactin® 4Flow®
Bead structure	4% agarose, crosslinked	6% agarose, crosslinked	4% agarose, highly crosslinked
Bead size	45-165 um	60-160 um	50-150 um, spherical
Exclusion limit	~3 x 10^7 Da	6 x 10^6 Da	3 x 10^7 Da
Recommended flow rate	gravity flow	0.5-1 ml/min	0.5-1 ml/min
pH range for protein binding	7-8	7-8	7-8
Max pressue	gravity flow	9.6 bar	3.5 bar
Eluent	desthiobiotin	desthiobiotin	desthiobiotin
Regeneration buffer	Buffer R	Buffer R	100 mM NaOH

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