BioLock Biotin Blocking Solution, 50 ml

Product Details

Catalog Number: 2-0205-050

Type: Blocking Buffer

Storage: Aliquot and store at -20°C or aliquot under sterile conditions and store at room temperature.

Shipping: Frozen (Polar Packs)

Species Reactivity: Not Applicable

Downloads: Datasheet (pdf)

Downloads: MSDS (pdf)

Product Sizes

Size	List Price	Price	Cart
50 ml		$275.00	Add to Cart

Cultivation media often contain significant amounts of biotin. This is especially the case for mammalian or insect cell culture media.

Thus, if proteins from biotin containing extracts or media are intended to be purified via Strep-Tactin® chromatography, biotin must be masked by the addition of avidin prior to the application onto the column. BioLock biotin blocking solution allows this masking of biotin (and biotinylated proteins) in a very fast and convenient way.

Activity: >70U/ml

Add at least 1U of BioLock solution per µg of biotin.

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Product Publications

Kwiwan Jeong, et al. (2022). Rapid Discovery and Classification of Inhibitors of Coronavirus Infection by Pseudovirus Screen and Amplified Luminescence Proximity Homogeneous Assay. Antiviral Research, 209:105473. doi: 10.1016/j.antiviral.2022.105473
Can Baysal, et al. (2022). Functional Expression of the Nitrogenase Fe Protein in Transgenic Rice. Communications Biology, 5:1006. doi: 10.1038/s42003-022-03921-9
J. Maximilian Fels, et al. (2021). Protective Neutralizing Antibodies from Human Survivors of Crimean-Congo Hemorrhagic Fever. Cell, 184(13), 3486-3501.E21. doi: 10.1016/j.cell.2021.05.001
Kuo Zhang, et al. (2021). Structural Insights into Viral RNA Capping and Plasma Membrane Targeting by Chikungunya Virus Nonstructural Protein 1. Cell Host & Microbe, 29(5), 757-764. doi: 10.1016/j.chom.2021.02.018
Emma C. Thomson, et al. (2021). Circulating SARS-CoV-2 Spike N439K Variants Maintain Fitness while Evading Antibody-Mediated Immunity. Cell, 184(5), 1171-1187.E20. doi: 10.1016/j.cell.2021.01.037
Kerstin Wernike, et al. (2021). Differentiation of Antibodies against Selected Simbu Serogroup Viruses by a Glycoprotein Gc-Based Triplex ELISA. Veterinary Sciences, 8(1), 12. doi: 10.3390/vetsci8010012
Antoni G. Wrobel, et al. (2020). SARS-CoV-2 and Bat RaTG13 Spike Glycoprotein Structures Inform on Virus Evolution and Furin-Cleavage Effects. Nature Structural & Molecular Biology, 27, 763–767. doi: 10.1038/s41594-020-0468-7
Juliette Hordeaux. (2019). The GPI-Linked Protein LY6A Drives AAV-PHP.B Transport across the Blood-Brain Barrier. Molecular Therapy, 27(5), 912-921. doi: 10.1016/j.ymthe.2019.02.013
Neeti Ananthaswamy, et al. (2019). A Sequestered Fusion Peptide in the Structure of an HIV-1 Transmitted Founder Envelope Trimer. Nature Communications, 10:873. doi: 10.1038/s41467-019-08825-7
Claudia Ortega, et al. (2018). Multi-Compartment and Multi-Host Vector Suite for Recombinant Protein Expression and Purification. Frontiers in Microbiology, 9. doi: 10.3389/fmicb.2018.01384
Watee Seesuay, et al. (2018). Human Transbodies that Interfere with the Functions of Ebola Virus VP35 Protein in Genome Replication and Transcription and Innate Immune Antagonism. Emerging Microbes & Infections, 7(1), 1-15. doi: 10.1038/s41426-018-0031-3
Wadad Al Salmi, et al. (2015). A New Approach to Produce HIV-1 Envelope Trimers. Journal of Biological Chemistry, 290(32), 19780-19795. doi: 10.1074/jbc.M115.656611

If you've used this product in a publication, let us know. Email pshuster@neuromics.com, with the publication details and you could be eligible for an Amazon gift card.

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Anhydrotetracycline	2-0401-002	25 mg
Desthiobiotin	2-1000-002	1 g
Desthiobiotin	2-1000-005	5 g
Desthiobiotin	2-1000-005	5 x 5 g
GFP-Strep-tag control protein	2-1006-005	500 ug
GFP-Twin-Strep-tag control protein	2-1007-105	500 ul
Strep-tag II Desthiobiotin elution reagent	2-1000-025	25 ml
Strep-tag II Peptide	2-1018-002	1.8 mg
Strep-tag II Peptide	2-1018-002	5 x 1.8 mg
Strep-tag regeneration buffer with HABA	2-1002-100	100 ml
Strep-tag washing buffer	2-1003-100	100 ml
WET FRED For 1 ml Gravity flow columns	2-0911-001	1 Set
WET FRED For 5-10 ml Gravity flow columns	2-0910-001	1 Set

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