Gravity Flow Strep-Tactin® 4Flow® High Capacity Column, 1 ml

Product Details

Catalog Number: 2-1252-001

Applications: Protein Purification, Gravity Flow

Storage: 2-8°C

Shipping: Room Temperature

Product Sizes

Size	List Price	Price	Cart
1 ml		$149.00	Add to Cart
5 x 1 ml		$710.00	Add to Cart

On 10/31/2024, Strep-Tactin® Sepharose® and Strep-Tactin® Superflow® products were discontinued. The replacement product is Strep-Tactin® 4Flow® High Capacity Products. Learn more below.

Strep-Tactin® 4Flow® high capacity columns are designed for purifying Strep-tag®II and Twin-Strep-tag® proteins via affinity chromatography in gravity flow. This product features a 4% cross-linked agarose beads coupled with Strep-Tactin®, a variant of streptavidin. Strep-Tactin® selectively binds to proteins fused with a Strep-tag®II or Twin-Strep-tag® and enables targeted elution via desthiobiotin. It can be regenerated with 100 mM NaOH for multiple uses, offering a cost-effective solution. This product serves as an alternative to our Strep-Tactin®XT 4Flow® resins for proteins that are challenging to elute.

Replacing:

Name	Catalog Number
Strep-Tactin® Sepharose® Column	2-1202-001
Strep-Tactin® Superflow® High Capacity Column	2-1209-001

Advantages of Strep-Tactin® 4Flow® High Capacity vs. Strep-Tactin® Sepharose® and Strep-Tactin® Superflow®:

Matrix	Strep-Tactin® Sepharose®	Strep-Tactin® Superflow®	Strep-Tactin® 4Flow®
Bead structure	4% agarose, crosslinked	6% agarose, crosslinked	4% agarose, highly crosslinked
Bead size	45-165 um	60-160 um	50-150 um, spherical
Exclusion limit	~3 x 10^7 Da	6 x 10^6 Da	3 x 10^7 Da
Recommended flow rate	gravity flow	0.5-1 ml/min	0.5-1 ml/min
pH range for protein binding	7-8	7-8	7-8
Max pressue	gravity flow	9.6 bar	3.5 bar
Eluent	desthiobiotin	desthiobiotin	desthiobiotin
Regeneration buffer	Buffer R	Buffer R	100 mM NaOH

Product Highlights:

IBA's New Protein Purification Resin

Product Reviews
- - Rating:
LEAVE A REVIEW
Leave a review for this product and you could be eligible for a $10 Starbucks gift card.
Product Publications
Product Publications
If you've used this product in a publication, let us know. Email pshuster@neuromics.com, with the publication details and you could be eligible for an Amazon gift card.

Related Products

Name	Catalog #	Size
10x Buffer BXT; Strep-Tactin®XT Elution Buffer	2-1042-025	25 ml
Avidin	2-0204-050	50 mg
BioLock Biotin blocking solution	2-0205-050	50 ml
BioLock Biotin blocking solution	2-0205-250	250 ml
Buffer XT-R	2-1045-250	250ml
D-Biotin	2-1016-002	2 g
D-Biotin	2-1016-005	10 x 5 g
D-Biotin	2-1016-005	5 g
Desthiobiotin	2-1000-002	1 g
Desthiobiotin	2-1000-005	5 x 5 g
Desthiobiotin	2-1000-005	5 g
Empty Spin Columns	2-5150-050	50 Columns
GFP-Strep-tag control protein	2-1006-005	500 ug
GFP-Twin-Strep-tag control protein	2-1007-105	500 ul
Magnetic Separator	2-1602-000	1 Piece
MagStrep "type3" Strep-Tactin® beads	2-1613-002	2 ml
MagStrep "type3" XT beads 5% suspension	2-4090-010	10 ml
MagStrep "type3" XT beads 5% suspension	2-4090-010	10 x 10 ml
MagStrep Strep-Tactin®XT Beads	2-5090-002	2 ml
MagStrep Strep-Tactin®XT Beads	2-5090-010	10 ml
MagStrep Strep-Tactin®XT Beads	2-5090-010	10 x 10 ml
Strep-Tactin® 4Flow® High Capacity Column	2-1251-005	5 x 0.2 ml
Strep-Tactin® 4Flow® High Capacity Column	2-1253-001	5 ml
Strep-Tactin® 4Flow® High Capacity Column	2-1253-001	5 x 5 ml
Strep-Tactin® 4Flow® High Capacity FPLC Column	2-1257-001	1 ml
Strep-Tactin® 4Flow® High Capacity FPLC Column	2-1257-001	10 x 1 ml
Strep-Tactin® 4Flow® High Capacity FPLC Column	2-1258-001	5 ml
Strep-Tactin® 4Flow® High Capacity FPLC Column	2-1258-001	10 x 5 ml
Strep-Tactin® 4Flow® High Capacity Resin	2-1250-002	4 ml
Strep-Tactin® 4Flow® High Capacity Resin	2-1250-010	5 x 20 ml
Strep-Tactin® 4Flow® High Capacity Resin	2-1250-010	20 ml
Strep-Tactin® 4Flow® High Capacity Resin	2-1250-025	10 x 50 ml
Strep-Tactin® 4Flow® High Capacity Resin	2-1250-025	50 ml
Strep-Tactin® 4Flow® High Capacity Resin	2-1250-025	5 x 50 ml
Strep-Tactin® MacroPrep® resin	2-1505-010	5 x 20 ml
Strep-Tactin® MacroPrep® resin	2-1505-010	20 ml
Strep-Tactin®XT 4Flow® cartridge	2-5023-001	10 x 1 ml
Strep-Tactin®XT 4Flow® cartridge	2-5023-001	1 ml
Strep-Tactin®XT 4Flow® cartridge	2-5024-001	10 x 5 ml
Strep-Tactin®XT 4Flow® cartridge	2-5024-001	5 ml
Strep-Tactin®XT 4Flow® column	2-5011-005	5 x 0.2 ml
Strep-Tactin®XT 4Flow® column	2-5012-001	5 x 1 ml
Strep-Tactin®XT 4Flow® column	2-5012-001	1 ml
Strep-Tactin®XT 4Flow® column	2-5013-001	5 x 5 ml
Strep-Tactin®XT 4Flow® column	2-5013-001	5 ml
Strep-Tactin®XT 4Flow® column	2-5014-001	10 ml
Strep-Tactin®XT 4Flow® high capacity cartridge	2-5027-001	10 x 1 ml
Strep-Tactin®XT 4Flow® high capacity cartridge	2-5027-001	1 ml
Strep-Tactin®XT 4Flow® high capacity cartridge	2-5028-001	5 ml
Strep-Tactin®XT 4Flow® high capacity cartridge	2-5028-001	10 x 5 ml
Strep-Tactin®XT 4Flow® high capacity column	2-5031-005	5 x 0.2 ml
Strep-Tactin®XT 4Flow® high capacity column	2-5032-001	1 ml
Strep-Tactin®XT 4Flow® high capacity column	2-5032-001	5 x 1 ml
Strep-Tactin®XT 4Flow® high capacity column	2-5033-001	5 x 5 ml
Strep-Tactin®XT 4Flow® high capacity column	2-5033-001	5 ml
Strep-Tactin®XT 4Flow® high capacity column	2-5034-001	10 ml
Strep-Tactin®XT 4Flow® high capacity resin	2-5030-002	4 ml
Strep-Tactin®XT 4Flow® high capacity resin	2-5030-010	5 x 20 ml
Strep-Tactin®XT 4Flow® high capacity resin	2-5030-010	20 ml
Strep-Tactin®XT 4Flow® high capacity resin	2-5030-025	10 x 50 ml
Strep-Tactin®XT 4Flow® high capacity resin	2-5030-025	50 ml
Strep-Tactin®XT 4Flow® high capacity resin	2-5030-025	5 x 50 ml
Strep-Tactin®XT 4Flow® high capacity Spin Column Kit	2-5151-000	1 Kit
Strep-Tactin®XT 4Flow® resin	2-5010-002	4 ml
Strep-Tactin®XT 4Flow® resin	2-5010-010	5 x 20 ml
Strep-Tactin®XT 4Flow® resin	2-5010-010	20 ml
Strep-Tactin®XT 4Flow® resin	2-5010-025	5 x 50 ml
Strep-Tactin®XT 4Flow® resin	2-5010-025	10 x 10 ml
Strep-Tactin®XT 4Flow® resin	2-5010-025	50 ml
Strep-Tactin®XT 4Flow® Starter Kit	2-5998-000	1 kit
Strep-Tactin®XT BLI Coupling Kit	2-4380-000	1 Kit
Strep-tag II Desthiobiotin elution reagent	2-1000-025	25 ml
Strep-tag II Peptide	2-1018-002	5 x 1.8 mg
Strep-tag II Peptide	2-1018-002	1.8 mg
Strep-tag regeneration buffer with HABA	2-1002-100	100 ml
Strep-tag washing buffer	2-1003-100	100 ml
WET FRED For 1 ml Gravity flow columns	2-0911-001	1 Set
WET FRED For 5-10 ml Gravity flow columns	2-0910-001	1 Set

Products

Customer Publications

Ordering Info & Policy

Blog Posts

Strep-Tactin® 4Flow® High Capacity Column

Product Details

Product Sizes

Product Publications

Related Products

Images