Gravity Flow Strep-Tactin® 4Flow® High Capacity Column, 5 x 0.2 ml

Product Details

Catalog Number: 2-1251-005

Applications: Protein Purification, Gravity Flow

Storage: 2-8°C

Shipping: Room Temperature

Product Sizes

Size	List Price	Price	Cart
5 x 0.2 ml		$333.00	Add to Cart

On 10/31/2024, Strep-Tactin® Sepharose® and Strep-Tactin® Superflow® products will be discontinued and replaced with the new and improved Strep-Tactin® 4Flow® High Capacity Products. Learn more below.

Strep-Tactin® 4Flow® high capacity columns are designed for purifying Strep-tag®II and Twin-Strep-tag® proteins via affinity chromatography in gravity flow. This product features a 4% cross-linked agarose beads coupled with Strep-Tactin®, a variant of streptavidin. Strep-Tactin® selectively binds to proteins fused with a Strep-tag®II or Twin-Strep-tag® and enables targeted elution via desthiobiotin. It can be regenerated with 100 mM NaOH for multiple uses, offering a cost-effective solution. This product serves as an alternative to our Strep-Tactin®XT 4Flow® resins for proteins that are challenging to elute.

Replacing:

Name	Catalog Number
Strep-Tactin® Superflow® High Capacity Column	2-1209-550

Advantages of Strep-Tactin® 4Flow® High Capacity vs. Strep-Tactin® Sepharose® and Strep-Tactin® Superflow®:

Matrix	Strep-Tactin® Sepharose®	Strep-Tactin® Superflow®	Strep-Tactin® 4Flow®
Bead structure	4% agarose, crosslinked	6% agarose, crosslinked	4% agarose, highly crosslinked
Bead size	45-165 um	60-160 um	50-150 um, spherical
Exclusion limit	~3 x 10^7 Da	6 x 10^6 Da	3 x 10^7 Da
Recommended flow rate	gravity flow	0.5-1 ml/min	0.5-1 ml/min
pH range for protein binding	7-8	7-8	7-8
Max pressue	gravity flow	9.6 bar	3.5 bar
Eluent	desthiobiotin	desthiobiotin	desthiobiotin
Regeneration buffer	Buffer R	Buffer R	100 mM NaOH

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