Strep-Tactin® 4Flow® High Capacity Resin, 4 ml

Product Details

Catalog Number: 2-1250-002

Applications: Protein Purification

Storage: 2-8°C

Shipping: Room Temperature

Format A: liquid

Product Sizes

Size	List Price	Price	Cart
4 ml		$200.00	Add to Cart

On 10/31/2024, Strep-Tactin® Sepharose® and Strep-Tactin® Superflow® products will be discontinued and replaced with the new and improved Strep-Tactin® 4Flow® High Capacity Products. Learn more below.

Strep-Tactin® 4Flow® high capacity is a resin for purification of strep-tagged proteins via affinity chromatography. The product is optimized for column-based use and suitable for HPLC/FPLC. It consists out of cross-linked agarose beads that are coupled to Strep-Tactin®, which is a streptavidin variant. Strep-Tactin® binds to proteins that were fused to a Strep-tag®II or a Twin-Strep-tag®, enabling a straightforward purification of tagged proteins from mixed samples, reaching a purity of >95%. Due to the targeted elution, tedious titration steps are not necessary. In addition, regeneration allows for multiple use of the resin. This resin is an alternative for proteins that are difficult to elute from our Strep-Tactin®XT 4Flow® resins.

Replacing:

Name	Catalog Number
Strep-Tactin® Sepharose® Resin	2-1201-002
Strep-Tactin® Superflow® High Capacity Resin	2-1208-002

Advantages of Strep-Tactin® 4Flow® High Capacity vs. Strep-Tactin® Sepharose® and Strep-Tactin® Superflow®:

Matrix	Strep-Tactin® Sepharose®	Strep-Tactin® Superflow®	Strep-Tactin® 4Flow®
Bead structure	4% agarose, crosslinked	6% agarose, crosslinked	4% agarose, highly crosslinked
Bead size	45-165 um	60-160 um	50-150 um, spherical
Exclusion limit	~3 x 10^7 Da	6 x 10^6 Da	3 x 10^7 Da
Recommended flow rate	gravity flow	0.5-1 ml/min	0.5-1 ml/min
pH range for protein binding	7-8	7-8	7-8
Max pressue	gravity flow	9.6 bar	3.5 bar
Eluent	desthiobiotin	desthiobiotin	desthiobiotin
Regeneration buffer	Buffer R	Buffer R	100 mM NaOH

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