Strep-Tactin® Superflow® high capacity cartridge, 1 ml

Product Details

Catalog Number: 2-1239-001

Applications: Protein Purification

Storage: 2 - 8 °C

Shipping: Room Temperature

Product Sizes

Size	List Price	Price	Cart

As of 10/31/2024, Strep-Tactin® Sepharose® products have been discontinued. The replacement products are Strep-Tactin® 4Flow® High Capacity Products.

Replacement:

Name	Catalog Number
Strep-Tactin® 4Flow® High Capacity FPLC Column	2-1257-001

Advantages of Strep-Tactin® 4Flow® High Capacity vs. Strep-Tactin® Superflow®:

Matrix	Strep-Tactin® Superflow®	Strep-Tactin® 4Flow®
Bead structure	6% agarose, crosslinked	4% agarose, highly crosslinked
Bead size	60-160 um	50-150 um, spherical
Exclusion limit	6 x 10^6 Da	3 x 10^7 Da
Recommended flow rate	0.5-1 ml/min	0.5-1 ml/min
pH range for protein binding	7-8	7-8
Max pressue	9.6 bar	3.5 bar
Eluent	desthiobiotin	desthiobiotin
Regeneration buffer	Buffer R	100 mM NaOH

Strep-Tactin® Superflow® high capacity cartridge for one-step purification of recombinant Strep-tag® proteins with HPLC or FPLC.

The cartridges are primarily designed for use with chromatography workstations with 10-32 connections (please note that you do not need adapters for HPLC and Äkta). They can, however, also be operated with other workstations, with syringes or with peristaltic pumps by use of common adapters for cartridges. Since column bodies are pressure resistant ) for up to 3 bar, the cartridges can be used with a flow restrictor. Cartridges can be connected in series to enlarge capacity.

Dynamic binding capacity: 7mg protein/ml resin, determined with mCherry-Twin-Strep-tag® (28kDa)

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