Product Details
Catalog Number: RA24288
Applications: WB, IHC
Type: Rabbit IgG
Dilutions: IHC 1:1000-1:2000
Immunogen: multiple antigenic peptide of an N-terminal synthetic sequence corresponding to amino acids 22-41 of rat 5HT2A receptor
Storage: Store at 4°C. Avoid freeze-thaw cycles.
Shipping: Refrigerate (Polar Packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Rat
Entrez: 3356
UniProt: P28223
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
100 ul$467.50Add to Cart

The affinity purified histochemical antibody for 5HT2A receptor was generated in rabbit against a multiple antigenic peptide of an N-terminal synthetic sequence corresponding to amino acids 22-41 of rat 5HT2A receptor.

The antibody produces 4+ (maximum) immunolabeling of 5HT2A receptor at dilutions of 1/300 - 1/500 using biotin-streptavidin/HRP technique with free-floating methods on rat cortex, amygdala and hippocampus. The addition of intensifying reagents such as nickel ammonium sulfate to the chromogen solution allows further dilution to approximately 1/1,000 or more. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. Immunolabeling is completely abolished by preadsorption with synthetic rat 5HT2A receptor (22-41). Immunolabeling of Western blot revealed a single band of approximately between 55 and 75 kDA. For Western Blot Protocol See: DOI:10.1124/jpet.111.18378.

Product Highlights:

Images
5HT2AR staining of rat cerebral cortex.

5HT2AR staining of rat cerebral cortex.

Mouse frontal cortical neuron cultures (p1 pups, day 4 culture stained-Neuromicsantibody: 5-HT2AR at 1:100 48 hours. MAP2 Ab: 1:20,000, 24 h. Details in Schmid, Raehal, and Bohn; 2008; PNAS

Mouse frontal cortical neuron cultures (p1 pups, day 4 culture stained-Neuromicsantibody: 5-HT2AR at 1:100 48 hours. MAP2 Ab: 1:20,000, 24 h. Details in Schmid, Raehal, and Bohn; 2008; PNAS

Questions?
We can help.

We are building our company one satisfied customer at a time. If you have any questions or concerns, please contact us.

Neuromics
5325 West 74th St., Suite 8
Edina, MN 55439

Toll free: 866-350-1500
Int’l phone: 952-374-6161
Fax: 612-677-3976