Product Details
Product Sizes
| Size | List Price | Price | Cart |
|---|---|---|---|
| 100 ul | $325.00 | Add to Cart |
mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in transfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kDa protein isolated originally from the jellyfish Aequoria victoria. The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma. DsRed is similar in size and properties to GFP, but, obviously, produces a red rather than a green fluorochrome. Several further cycles of mutation, directed modification and evolutionary selection produced mCherry, which has an excitation maximum at 587 nm and and emission maximum at 610 nm (4). We expressed the mCherry protein sequence shown in reference 4 in bacteria, purified out the mCherry and raised this rabbit polyclonal antibody. This was affinity purified and was found to stain a band of the expected size in HEK293 cells transfected with the pFin-EF1-mCherry vector designed to express mCherry. As shown below, the antibody does not stain any protein band in untransfected HEK293 cells. |
- Product Reviews- - Rating:
Leave a review for this product and you could be eligible for a $10 Starbucks gift card.
- Product Publications
Product Publications
If you've used this product in a publication, let us know. Email pshuster@neuromics.com, with the publication details and you could be eligible for an Amazon gift card.
- Related Products
Related Products
Name Catalog # Size ATPase Na+/K+ transporting alpha 1 MO25023 50 ul Fibrillarin CH22129 100 ul Fibrillarin MO22169 100 µl Fibrillarin RA22147 100 ul Fibrillarin/Nop1p- Nucleolar Marker MO22108 500 ul FluoGreen Tracer SF41000 100 ul GFP GT22101 100 ul Green Fluorescent Protein CH22124 100 ul of 1 mg/ml Green Fluorescent Protein MO22184 100 ul Green Fluorescent Protein (GFP) MO22190 100 ul Green Fluorescent Protein (GFP) RA22134 100 ul of 1 mg/ml High-mobility group protein B1/HMGP1 MO22134 100 ul Lamin A/C CH22136 100 ul Lamin A/C MO22124 100 ul mCherry GT22108 100 ul mCherry MO22192 100 ul mCherry - Mouse MO22140 100 ul mCherry-Chicken CH22115 100 ul MiTF (Microphthalmia-Associated Transcription Factor) MO47034 0.5 ml Panspecific Nuclear Pore Complex Marker MO22107 500 ul S-Tag/S15 MO22203 100 ul S-Tag/S15 MO22206 100 ul TAF15 MO22114 100 ul Tar DNA Binding Protein 43 (TDP43) MO22117 100 ul
Images
HEK293 cells transfected in the same way and viewed in the confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are red. Staining with mCherry (RA22117) is shown in Green. courtesy of the Semple-Rowland lab at the University of Florida.
HEK293 cells transfected in the same way and viewed in the confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are red. Staining with mCherry (RA22117) is shown in Green. courtesy of the Semple-Rowland lab at the University of Florida.