CD146

CD146 (433-647 a.a.) belongs to the immunoglobulin supergene family with five immunoglobulin like domains (V-V-C2-C2-C2), a transmembrane region and a 63 residue cytoplasmic tail. The protein is a membrane glycoprotein that functions as a Ca2+ independent cell adhesion molecule involved in heterophilic cell to cell interactions. CD146 has a molecular size of 130 kDa in its reduced form
(118 kDa unreduced), and N linked glycosylation accounts for fifty percent of the apparent molecular weight. In some cells the molecule carries a sulfate 3 glucuronyl moiety.

Applications:

• ELISA
• Inhibition Assays
• Western Blotting-On SDS-PAGE commassie blue stained gel, the purified recombinant protein shows a band at 47 kDa.

References:

1. Role of CD146 in detection of apoptotic circulating endothelial cells in obstructive sleep apnea. Am J Respir Crit Care Med 2007 Jun 15;175(12):1346-1347; author reply 1347
2. Elevation of serum adiponectin and CD146 levels in diabetic nephropathy. Diabetes Res Clin Pract 2007 May 8.
3. Restoration of soluble CD146 in patients with Crohn's disease treated with the TNF-alpha antagonist infliximab. Inflamm Bowel Dis 2007 May 2.
4. Visualization of CD146 dimerization and its regulation in living cells. Biochim Biophys Acta 2007 Apr;1773(4):513-20.
5. Poor prognosis in breast carcinomas correlates with increased expression of targetable CD146 and c-Met and with proteomic basal-like phenotype. Hum Pathol 2007 Jun;38(6):830-41
6. Detection of circulating endothelial cells: CD146-based magnetic separation enrichment or flow cytometric assay? J Clin Oncol 2007 Feb 10;25(5):e1-2; author reply e3-5