Product Details
Catalog Number: MO22138
Applications: ICC, WB, IHC, IF
Type: Mouse IgG
Immunogen: Full length recombinant human Aurora B protein expressed in and purified from E. coli.
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Protein G Purified
Format B: liquid
Species Reactivity: Human, Mouse, Rat, Chicken, Bovine, Porcine
Entrez: 9212
UniProt: Q96GD4
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$325.00Add to Cart

Aurora proteins are a family of proteins that play a key role in the regulation of cell division.

Images
HeLa cell cultures were stained with Aurora B kinase antibody (green)

HeLa cell cultures were stained with Aurora B kinase antibody (green). Aurora B stains midzones in anaphase and midbodies between the two daughter cells during telophase. It is therefore a useful marker of dividing cells. Counterstained is our chicken polyclonal antibody against Vimentin, (red, CH22108). Blue is a DNA stain.

HeLa cell cultures were stained with Aurora B kinase antibody (green)

HeLa cell cultures were stained with Aurora B kinase antibody (green). Aurora B stains midzones in anaphase and midbodies between the two daughter cells during telophase. It is therefore a useful marker of dividing cells. Counterstained is our chicken polyclonal antibody against Vimentin, (red, CH22108). Blue is a DNA stain.

Western blot analysis of Aurora B in HeLa cells.

Left: Western blot analysis of Aurora B in HeLa cells. Blot of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with Aurora B. The Aurora B monoclonal binds strongly to aurora B at 38 kDa. Right: Blot of recombinant human Aurora A, B and C were probed with MO22138. This antibody binds specifically to Aurora B. This is also consisted with the immunocytochemical staining of midzones and midbodies on HeLa cells.

Western blot analysis of Aurora B in HeLa cells.

Left: Western blot analysis of Aurora B in HeLa cells. Blot of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with Aurora B. The Aurora B monoclonal binds strongly to aurora B at 38 kDa. Right: Blot of recombinant human Aurora A, B and C were probed with MO22138. This antibody binds specifically to Aurora B. This is also consisted with the immunocytochemical staining of midzones and midbodies on HeLa cells.

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