Product Details
Catalog Number: HEC09-T25
Applications: Cell Assays
Type: Primary Cells
Storage: 37°C Incubator
Species Reactivity: Human
Product Sizes
SizeList PricePriceCart
500,000+ Cells in T25 Flask$995.00Add to Cart

HRMECs were initiated by elutriation from dissociated normal human retinal tissue. Passage 1 cells are ship in proliferating culture with a confluency of >90 %*. ENDO-Growth medium is recommended for culture. Cells have an average additional population doubling levels >15 when cultured.

Characterization of the cells

  • Cytoplasmic VWF / Factor VIII: >95% positive by immunofluorescence
  • Cytoplasmic uptake of Di-I-Ac-LDL: >95% positive by immunofluorescence
  • Cytoplasmic PECAM1 >95% positive by immunofluorescence
HRMECs are negative for HIV-1, HBV, HCV, and mycoplasma.

*cells are shipped at 90% confluence. However, confluence can be lost during shipping. Please allow for 1-4 days to reach 90% confluence again. As the cells are shipped live, extreme weather (including extreme cold or heat) can be a factor if we are able to ship or not. 

Cells are prepared and manufactured in an ISO 9001:2015 certified environment and ISO 5 cleanroom.

Product Highlights:
Supporting Products
NameCatalog #Size
1X Phosphate Buffer SolutionPBS300100 ml
AlphaBioCoat Precoated 100 mm DishesAC001-100MMPack of 10
AlphaBioCoat Precoated 12-Well PlatesAC001-12Pack of 10
AlphaBioCoat Precoated 150 mm DishesAC001-150MMPack of 10
AlphaBioCoat Precoated 24-Well PlatesAC001-24Pack of 10
AlphaBioCoat Precoated 35 mm DishesAC001-35MMPack of 10
AlphaBioCoat Precoated 48-Well PlatesAC001-48Pack of 10
AlphaBioCoat Precoated 6-Well PlatesAC001-6Pack of 10
AlphaBioCoat Precoated 60 mm DishesAC001-60MMPack of 10
AlphaBioCoat Precoated 96-Well PlatesAC001-96Pack of 10
AlphaBioCoat Precoated T-175 FlasksAC001-T175Pack of 10
AlphaBioCoat Precoated T-25 FlasksAC001-T25Pack of 10
AlphaBioCoat Precoated T-75 FlasksAC001-T75Pack of 10
AlphaBioCoat SolutionAC00120 ml
Cell Detachment SolutionADF00150 ml
ENDO Basal MediaMED002500 ml
ENDO-Growth MediaMED001500 ml
ENDO-Growth Media KitEGK001500 ml
ENDO-Growth SupplementsEGS00125 ml
Endothelial Cryopreserve MediaFM30010 ml
Epithelial Cryopreserve MediaFM30120 ml
Human Epithelial Cell Basal MediumECM002500 ml
Human Epithelial Cell MediumECM001500 mL
Smooth Coat SolutionSC30020 ml
Universal Tube Formation MediaMED003500 ml
Images
HREMCs

Effect of HG (high glucose) and pro-inflammatory cytokines on connexin43 expression in HRMECs. Immunohistochemical data showing connexin43 expression in (A) normal medium; (B) HG (25 mM); (C) pro-inflammatory cytokines (IL-1β and TNF-α 10 ng/mL each); and (D) a combination of HG and pro-inflammatory cytokines inducing a change in cell morphology with signs of cell swelling, possibly owing to hemichannel opening (indicated by white arrows).

HREMCs

Effect of HG (high glucose) and pro-inflammatory cytokines on connexin43 expression in HRMECs. Immunohistochemical data showing connexin43 expression in (A) normal medium; (B) HG (25 mM); (C) pro-inflammatory cytokines (IL-1β and TNF-α 10 ng/mL each); and (D) a combination of HG and pro-inflammatory cytokines inducing a change in cell morphology with signs of cell swelling, possibly owing to hemichannel opening (indicated by white arrows).

Retinal Microvascular Endothelial Cells in culture

Retinal Microvascular Endothelial Cells in culture.

Retinal Microvascular Endothelial Cells in culture

Retinal Microvascular Endothelial Cells in culture.

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©2024 Neuromics. All rights reserved.