Human Retinal Microvascular Endothelial Cells or HRMECS

Product Details

Catalog Number: HEC09

Applications: Cell Assays

Type: Primary Cells

Storage: Keep frozen in liquid nitrogen until plating

Shipping: Frozen (Dry Ice/Liquid Nitrogen)

Species Reactivity: Human

Downloads: CD31 and vWF Data (pdf)

Downloads: Datasheet (pdf)

Product Sizes

Size	List Price	Price	Cart
500,000+ Cells Frozen		$895.50	Add to Cart

HRMECs were initiated by elutriation from dissociated normal human retinal tissue. Passage 1 cells are shipped frozen. ENDO-Growth medium is recommended for culture. Cells have an average additional population doubling levels >15 when cultured.

Characterization of the cells

Cytoplasmic VWF / Factor VIII: >95% positive by immunofluorescence
Cytoplasmic uptake of Di-I-Ac-LDL: >95% positive by immunofluorescence
Cytoplasmic PECAM1 >95% positive by immunofluorescence

HRMECs are negative for HIV-1, HBV, HCV, and mycoplasma.

Cells are prepared and manufactured in an ISO 9001:2015 certified environment and ISO 5 cleanroom.

Product Highlights:

Supporting Products

Name	Catalog #	Size
1X Phosphate Buffer Solution	PBS300	100 ml
AlphaBioCoat Precoated 100 mm Dishes	AC001-100MM	Pack of 10
AlphaBioCoat Precoated 12-Well Plates	AC001-12	Pack of 10
AlphaBioCoat Precoated 150 mm Dishes	AC001-150MM	Pack of 10
AlphaBioCoat Precoated 24-Well Plates	AC001-24	Pack of 10
AlphaBioCoat Precoated 35 mm Dishes	AC001-35MM	Pack of 10
AlphaBioCoat Precoated 48-Well Plates	AC001-48	Pack of 10
AlphaBioCoat Precoated 6-Well Plates	AC001-6	Pack of 10
AlphaBioCoat Precoated 60 mm Dishes	AC001-60MM	Pack of 10
AlphaBioCoat Precoated 96-Well Plates	AC001-96	Pack of 10
AlphaBioCoat Precoated T-175 Flasks	AC001-T175	Pack of 10
AlphaBioCoat Precoated T-25 Flasks	AC001-T25	Pack of 10
AlphaBioCoat Precoated T-75 Flasks	AC001-T75	Pack of 10
AlphaBioCoat Solution	AC001	20 ml
Cas9 Endothelial Basal Media	MED005	500 ml
Cas9 Endothelial Growth Media	MED004	500 ml
Cell Detachment Solution	ADF001	50 ml
ENDO Basal Media	MED002	500 ml
ENDO-Growth Media	MED001	500 ml
ENDO-Growth Media Kit	EGK001	500 ml
ENDO-Growth Supplements	EGS001	25 ml
Endothelial Cryopreserve Media	FM300	10 ml
Epithelial Cryopreserve Media	FM301	20 ml
Human Epithelial Cell Basal Medium	ECM002	500 ml
Human Epithelial Cell Medium	ECM001	500 mL
Smooth Coat Solution	SC300	20 ml
Universal Tube Formation Media	MED003	500 ml

Product Reviews

Product Publications

Jiawei Wang, Nannan Yang, Wanna Li, Han Zhang, and Jianqiao Li. (2024). Role of Hsa_circ_0000880 in the Regulation of High Glucose-Induced Apoptosis of Retinal Microvascular Endothelial Cells. Translational Vision Science & Technology, 13:12. doi: 10.1167/tvst.13.4.12
Sergio Crespo-Garcia, Frédérik Fournier, Roberto Diaz-Marin, Sharon Klier, Derek Ragusa, Lauren Masaki, Gael Cagnone, Guillaume Blot, Ikhlas Hafiane, Agnieszka Dejda, Rana Rizk, Rachel Juneau, Manuel Buscarlet, Sarah Chorfi, Priyanka Patel, Pedro J. Beltran, Jean-Sebastien Joyal, Flavio A. Rezende, Masayuki Hata, Alex Nguyen, Lynne Sullivan, Jason Damiano, Ariel M. Wilson, Frédérick A. Mallette, Nathaniel E. David, Anirvan Ghosh, Pamela R. Tsuruda, Jamie Dananberg, and Przemyslaw Sapieha. (2024). Therapeutic Targeting of Cellular Senescence in Diabetic Macular Edema: Preclinical and Phase 1 Trial Results. Nature Medicine. doi: 10.1038/s41591-024-02802-4
Yong Yao Xu, Michael Twarog, Ning Li, Angela Banks, Josh Schustak, Yi Bao, Qian Huang, and Quintus Medley. (2022). Zinc Transport from the Endoplasmic Reticulum to the Cytoplasm via Zip7 is Necessary for Barrier Dysfunction Mediated by Inflammatory Signaling in RPE Cells. PLoS ONE 17(7): e0271656. doi: 10.1371/journal.pone.0271656
A. Gericke, K. Suminska-Jasinska, and A. Breborowicz. (2021). Sulodexide Reduces Glucose Induced Senescence in Human Retinal Endothelial Cells. Scientific Reports, 11:11532. doi: 10.1038/s41598-021-90987-w
Fei Cai, Huanzong Jiang, Yan Li, Qin Li, and Chao Yang. (2021). Up-Regulation of Long Non-Coding RNA SNHG16 Promotes Diabetes-Related Retinal Microvascular Endothelial Cell Dysfunction via Activating NF-κB and PI3K/AKT Pathways. Molecular Therapy: Nucleic Acid. doi: 10.1016/j.omtn.2021.01.035
A. P. Da Cunha, Q. Zhang, M. Prentiss, X. Q. Wu, V. Kainz, Y. Y. Xu, J. Vrouvlianis, H. Li, N. Rangaswamy, B. Leehy, T. L. McGee, C. L. Bell, C. E. Bigelow, V. Kansara, Q. Medley, Q. Huang & H. Y. Wu. The Hierarchy of Proinflammatory Cytokines in Ocular Inflammation. Current Eye Research, Volume 43, 2018 - Issue 4 Published Online: 04 Dec 2017
Odunayo O. Mugisho, Colin R. Green, Jie Zhang, Nicolette Binz, Monica L. Acosta, Elizabeth Rakoczy and Ilva D. Rupentha. (2017). Immunohistochemical Characterization of Connexin43 Expression in a Mouse Model of Diabetic Retinopathy and in Human Donor Retinas. Int. J. Mol. Sci. doi: 10.3390/ijms18122567
Michael Anthony Ruiz, Biao Feng, and Subrata Chakrabarti. (2015). Polycomb Repressive Complex 2 Regulates MiR-200b in Retinal Endothelial Cells: Potential Relevance in Diabetic Retinopathy. PLoS One.10(4): e0123987. doi: 10.1371/journal.pone.0123987.

Product Patent Application

Pam Tsuruda, Jill Hopkins, Harry Sweigard, Yan Poon, Jamie Dananberg, Daniel Marquess, and Nathaniel David. (2020). Methods of Inhibiting Pathological Angiogenesis. United States Patent Application 20200253991

If you've used this product in a publication, let us know. Email pshuster@neuromics.com, with the publication details and you could be eligible for an Amazon gift card.

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Images

Effect of HG (high glucose) and pro-inflammatory cytokines on connexin43 expression in HRMECs. Immunohistochemical data showing connexin43 expression in (A) normal medium; (B) HG (25 mM); (C) pro-inflammatory cytokines (IL-1β and TNF-α 10 ng/mL each); and (D) a combination of HG and pro-inflammatory cytokines inducing a change in cell morphology with signs of cell swelling, possibly owing to hemichannel opening (indicated by white arrows). https://doi.org/10.1080/02713683.2017.1410180

Retinal Microvascular Endothelial Cells in culture

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Human Retinal Microvascular Endothelial Cells