Human Brain Microvascular Endothelial Cells

HBMECs were isolated from normal human brain tissue. Passage 1, cells are ship on dry ice in a vial or in proliferating culture with a confluency of >90 %*.  ENDO-Growth medium containing 5% serum and growth supplements are recommended for culture. Cells have an average additional population doubling levels >15 when cultured.

• Cytoplasmic VWF / Factor VIII: >95% positive by immunofluorescence
• Cytoplasmic uptake of Di-I-Ac-LDL: >95% positive by immunofluorescence
• Cytoplasmic PECAM1 >95% positive by immunofluorescence 

HBMECs are negative for HIV-1, HBV, HCV, and mycoplasma.

*cells are shipped at 90% confluence. However, confluence can be lost during shipping. Please allow for 1-4 days to reach 90% confluence again. As the cells are shipped live, extreme weather (including extreme cold or heat) can be a factor if we are able to ship or not. 

Cells are prepared and manufactured in an ISO 9001:2015 certified environment and ISO 5 cleanroom.