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Size | List Price | Price | Cart |
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500,000+ Cells frozen | $983.88 | Add to Cart |
GFP expressing human umbilical vein endothelial cells were isolated from normal human umbilical vein and transfected with GFP-lentiviral particles at passage 1. Puromycin resistant GFP-HUVECs are passage 3 and are shipped frozen (or in proliferating culture with a confluency of >90 %, GF01-T25). ENDO-Growth Media (MED001) is recommended for culture. Cells have an average additional population doubling levels >18 when cultured. Characterization of the cells
HUVECs are negative for HIV-1, HBV, HCV, and mycoplasma. Cells are prepared and manufactured in an ISO 9001:2015 certified environment and ISO 5 cleanroom. Product Highlights: |
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- Product Publications
Product Publications
- Samuel Olson, McKenna Finley, and Raviraj Thakur. (2024). An Open-Source, Battery-Powered, Low-Cost, and Dual-Channel Pneumatic Pulse Generator for Microfluidic Cell-Stretch Assays. HardwareX, e00595. doi: 10.1016/j.ohx.2024.e00595
- Mahboobeh Mahmoodi, Mohammad Ali Darabi, Neda Mohaghegh, Ahmet Erdem, Amir Ahari, Reza Abbasgholizadeh, Maryam Tavafoghi, Paria Mir Hashemian, Vahid Hosseini, Javed Iqbal, Reihaneh Haghniaz, Hossein Montazerian, Jamileh Jahangiry, Fatemeh Nasrolahi, Arshia Mirjafari, Erik Pagan, Mohsen Akbari, Hojae Bae, Johnson V. John, Hossein Heidari, Ali Khademhosseini, and Alireza Hassani Najafabadi. (2024). Egg White Photocrosslinkable Hydrogels as Versatile Bioinks for Advanced Tissue Engineering Applications. Advanced Functional Materials, 2315040. doi: 10.1002/adfm.202315040
- Yan Li, Chuanlin Hu, Pengcheng Wang, Yan Liu, Luyang Wang, Qingmeng Pi, Zhiyong Gong, Xu Yang, Michael Mak and Yang Wu (2019). Indoor nanoscale particulate matter-induced coagulation abnormality based on a human 3D microvascular model on a microfluidic chip. Journal of Nanobiotechnology 2019 17:20. https://doi.org/10.1186/s12951-019-0458-2
- Yan Li, Qing-Meng Pi, Peng-Cheng Wang, Lie-Ju Liu, Zheng-Gang Han,Yang Shao, Ying Zhai, Zheng-Yu Zuo, Zhi-Yong Gong, Xu Yang and Yang Wu. (2017). Functional human 3D microvascular networks on a chip to study the procoagulant effects of ambient fine particulate matter. RSC Adv. 7, 56108
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Name Catalog # Size GFP Expressing Human Umbilical Vein Endothelial Cells GF01-T25 500,000+ Cells in T25 flask Human Umbilical Vein Endothelial Cells HEC01 500,000+ Cells frozen Human Umbilical Vein Endothelial Cells HEC01-T25 500,000+ Cells in T25 Flask
Images
GFP-HUVECs stained with VE-Cadherin and DAPI on an egg white based hydrogel.
GFP-HUVECs stained with VE-Cadherin and DAPI on an egg white based hydrogel.
IVSWT-mediated HUVEC vasculogenesis. (A) Overview of the treatment setup for stimulation of EC/ASC co-cultures in fibrin. (B) Fluorescent images of non-treated versus treated LEC and HUVEC networks on day 7. (C) Quantifications of HUVEC networks. IVSWT increased the number of junctions, tubules and the total tubule length. The mean tubule length was decreased. P-values: *** ≤0.01, ** ≤0.1, * ≤0.5. doi:10.1371/journal.pone.0114806.g002.
IVSWT-mediated HUVEC vasculogenesis. (A) Overview of the treatment setup for stimulation of EC/ASC co-cultures in fibrin. (B) Fluorescent images of non-treated versus treated LEC and HUVEC networks on day 7. (C) Quantifications of HUVEC networks. IVSWT increased the number of junctions, tubules and the total tubule length. The mean tubule length was decreased. P-values: *** ≤0.01, ** ≤0.1, * ≤0.5. doi:10.1371/journal.pone.0114806.g002.
Fluorescent imaging of GFP-HUVECs on days 1-4 seeded in various hydrogels.
Fluorescent imaging of GFP-HUVECs on days 1-4 seeded in various hydrogels.