Product Details
Catalog Number: 6-3201-002
Applications: Cell Assays
Type: Cell Isolation-Separation
Storage: Store all components at 2-8°C
Shipping: Ambient
Species Reactivity: Human
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
 

Discontinued

Fab-TACS® - Traceless Affinity Cell Selection - is a positive cell isolation approach based on our Strep-tag® technology. CD3 Fab-TACS® Agarose Column Starter Kit allows label-free isolation of human CD3+ T cells from whole blood, buffy coat, PBMCs or other cell suspensions. CD3 is a co-receptor that associates with the T cell receptor and plays a role in T cell activation.

Strep-Tactin® TACS Agarose columns are filled with Strep-Tactin® coated agarose macrobeads developed for cell isolation. Fab fragments fused to a Twin-Strep-tag® (Fab-Streps) bind to the matrix. Target cells adhere to the matrix based on the exclusive binding of the Fab-Strep to a surface marker. Non-target cells are efficiently washed away. In a final step, the addition of biotin causes the elution of the target cells and the Fab-Streps. After elution, the Fab-Streps self-dissociate from the cell surfaces due to their low affinity. The label-free authentic target cells are now ready for further downstream applications.

Advantages:

  • Highly pure cells directly from whole blood
  • Reversible binding to the target
  • Highly viable, non-activated cells
  • Label-free cells due to low affinity Fab fragments

Contents:

  • Strep-Tactin® TACS Agarose Columns
  • CD3 Fab-Strep, human
  • 10x Buffer CI
  • 100 mM Biotin stock solution
  • TACS Column Adapter
Images

Questions?
We can help.

We are building our company one satisfied customer at a time. If you have any questions or concerns, please contact us.

Neuromics
5325 West 74th St., Suite 8
Edina, MN 55439

Toll free: 866-350-1500
Int’l phone: 952-374-6161
Fax: 612-677-3976