pDSG-IBAwt2 vector

StarGate Acceptor Vector designed for high-level stable transient expression in most mammalian hosts containing the following elements:

• Human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells
• Episomal replication through Epstein Barr Virus replication origin (oriP) requires chromosomally expressed nuclear antigen encoded by EBNA-1
• Ampicillin resistance and ColE1 replication origin (pUC) for propagation in E. coli.
• Optimized for MEXi (Mammalian expression system IBA)

The expressed recombinant protein will be secreted in the cell culture medium (BM40 signal sequence).

This vector does not contain an affinity tag.