pCSG-IBA142 vector

Please note: This product will be discontinued on 12/31/2024

The pCSG-IBA142 vector allows high-level expression of Twin-Strep®/6xHistidine-tag-fusion-proteins in mammalian hosts under G418 (Geneticin) selection. The vector carries the Human cytomegalovirus (CMV) immediate-early promoter for high-level expression of proteins in a wide range of mammalian cells, the BM40 signal for secretion of the recombinant protein, the Twin-Strep-tag® for C-terminal and the 6xHistidine-tag for N-terminal fusion to the recombinant protein. The episomal replication is mediated through the Epstein Barr Virus replication origin (oriP) and nuclear antigen encoded by EBNA-1 in human, primate and canine cells and through SV40 replication origin in cells latently infected with SV40 or that express the SV40 large T antigen (e.g. COS-1, COS-7). For selection and propagation in E.coli the vector carries an Ampicillin resistance and ColE1 origin of replication (pUC). The expressed recombinant protein will be secreted into the medium.