pYSG-IBA164 vector

Please note: This product will be discontinued on 12/31/2024

The pYSG-IBA164 vector allows the high-level expression of Twin-Strep® /FLAG-tag-fusion-proteins in yeast. The vector carries the copper inducible promoter (CUP1) for controlled high-level expression, the Twin-Strep-tag® for N-terminal and the FLAG-tag for C-terminal fusion of the recombinant protein as well as the URA3 auxotrophy marker for selection after transformation (do not use URA3 for selection during expression), the LEU2d auxotrophy marker for selection to increase plasmid copy number for expression (do not use LEU2d for selection after transformation). The expressed recombinant protein will be localized in the cytoplasm.