pASG-IBA144

The pASG-IBA144 vector allows the expression of Twin-Strep®-/6xHistidine-tag-fusion-proteins in E.coli. The vector carries the inducible tetracycline promoter/operator for the regulated expression of proteins and the ompA signal for periplasmic secretion of the recombinant protein. Additionally it contains the Twin-Strep-tag® for N-terminal and the 6xHistidine-tag for C-terminal fusion to the recombinant protein as well as the Ampicillin Resistance cassette. It can be used with any E.coli strain because the tet-promoter works independently from the genetic background of E.coli.

StarGate® Acceptor Vector for bacterial expression.

• The expression cassette is under transcriptional control of the tetracycline promoter/operator.
• Compatible with any E. coli strain. The tet-promoter works independently from the genetic background of E. coli.
• The expressed recombinant protein will be secreted into the periplasm.