pASG-IBA2 vector

The new StarGate Combi Entry Cloning Set allows two types of Entry Cloning with one Set: 1. Entry Cloning of your gene of interest (GOI) into the pENTRY vector resulting in a Donor Vector carrying the inserted GOI. 2. Site-directed mutation of your gene of interest (GOI) by preparing two simple PCR reactions, followed by the cloning of the mutated gene into the pENTRY-IBA vector resulting in a Donor Vector with the mutated GOI The genes from the Donor Vectors can be transferred into a multitude of Acceptor Vectors carrying different tags/promoters for expression in different hosts. Create up to 20 different Donor Vectors with the StarGate Combi Entry Cloning Set.Includes:

• pENTRY-IBA vector (20 rxn) StarSolution M1, M2, M3 (20 rxn each)
• Competent E. coli TOP10 cells (20 rxn)
• For/Rev Sequencing primers
• DNA ruler
• Comprehensive manual

Note: To perform the complete StarGate® Cloning procedure, you require one of the Entry Cloning Sets (Standard or Mutagenesis) and StarGate® Transfer Reagent Set. For Fusion Cloning, you additionally require one of the Fusion Cloning Sets.