Twin-Strep-tag® Capture Kit

Twin-Strep-tag® Capture Kit is intended for site-directed, reversible capture of Twin-Strep-tag® fusion proteins for biomolecular interaction analysis using surface plasmon resonance (SPR) devices. The kit provides Strep-Tactin®XT, SPR Immobilization Buffer, Regeneration Buffer and GFP-Twin-Strep-tag® as control protein. It enables the highly stable immobilization of the ligand based on the unique Strep-Tactin®XT:Twin-Strep-tag® technology, due to the pM affinity of Twin-Strep-tag® to Strep-Tactin®XT.

The key benefits are:

• the indirect immobilization preventing the ligand for chemical modification as well as inactivation or manipulation by the immobilization procedure,
• the high affinity enabling measurements over a long time (high affinity in low pM range; T1/2 = 13 h)
• chip can be regenerated easily by an established procedure ? cost effective
• ligands can be captured directly from complex samples due to the high affinity and specificity of the Twin-Strep-tag®:Strep-Tactin®XT interaction

The use of unconjugated Strep-Tactin®XT protein requires a license that is provided with the purchase of the kit as limited use license.