pASK-IBA7C Protein Expression Vector or Plasmid

Catalog Number: 2-1326-000

Applications: Targeted Protein Expression

Type: Expression Vector

Species Reactivity: E. Coli

Size	List Price	Price	Cart
5 ug		$282.00	Add to Cart

Description	Expression plasmid. The expression cassette is under transcriptional control of the tetracycline promoter/operator. The expressed recombinant protein will be localized in the cytoplasm.
Affinity tag	Strep-Tactin® affinity tag (Strep-tag II®) for the purification of recombinant protein. The affinity tag is fused to the N-terminus of the recombinant protein and can be removed by cleavage with factor Xa.
Bacterial Expression	Expression is induced upon addition of 200 ug anhydrotetracycline (order no.: 2 0401 001; 2 0401 002) per 1 liter E. coli shaking culture (A₅₅₀= 0.5).
Expression strain	Any E. coli strain. The tet promoter works independently from the genetic background of E. coli.
Resistance	Chloramphenicol. Note: The CamR resistance gene codes for homotetrameric chloramphenicol acetyltransferase (MW of the monomer = 26.6 kDa) which is predominantly expressed in the cytosol of E.colitransformed with this plasmid
Form	5 ug, dissolved in 10 mM Tris/HCl pH 8.0, 1 mM EDTA; 20 ul.
Concentration	250 ng/ul.
Storage	4 °C for frequent usage, 20 °C for long term storage.

Product Reviews
- - Rating:
LEAVE A REVIEW
Leave a review for this product and you could be eligible for a $10 Starbucks gift card.
Product Publications
Product Publications
If you've used this product in a publication, let us know. Email pshuster@neuromics.com, with the publication details and you could be eligible for an Amazon gift card.
Related Products
Related Products
Name Catalog # Size
Competent cells E. coli TOP10 5-1600-020 20 rxns
pASK-IBA2C 2-1321-000 5 ug
pASK-IBA3C 2-1322-000 5 ug
pASK-IBA4C 2-1323-000 5 ug
pASK-IBA5C 2-1324-000 5 ug
pASK-IBA6C 2-1325-000 5 ug

Products