Product Details
Catalog Number: 2-1325-000
Applications: Targeted Protein Expression
Type: Expression Vector
Species Reactivity: E. Coli
Downloads: Datasheet (pdf)
Downloads: MSDS (pdf)
Product Sizes
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5 ug$282.00Add to Cart

Description

Expression plasmid. The expression cassette is under transcriptional control of the tetracycline promoter/operator. The expressed  recombinant protein will be secreted into the periplasm
Affinity tag

Strep-Tactin® affinity tag (Strep-tag II®) for the purification of recombinant protein. The affinity tag is fused to the N-terminus of the recombinant protein and can be removed by cleavage with factor Xa.

SecretionThe ompA signal sequence directs the expressed protein into the periplasmic space and will be cleaved off during the translocation process.
Bacterial Expression Expression is induced upon addition of  200 ug anhydrotetracycline (order no.: 2 0401 001; 2 0401 002) per 1 liter E. coli shaking culture (A550= 0.5).
Expression strain

Any E. coli strain. The tet promoter works independently from the genetic background of E. coli.

Resistance

Chloramphenicol. Note: The CamR resistance gene codes for homotetrameric chloramphenicol acetyltransferase (MW of the monomer = 26.6 kDa) which is predominantly expressed in the cytosol of E.coli transformed with this plasmid.

Form5 ug, dissolved in 10 mM Tris/HCl pH 8.0, 1 mM EDTA; 20 ul.
Concentration250 ng/ul.
Storage4 °C for frequent usage, 20 °C for long term storage.
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