pASK-IBA4C Protein Expression Vector or Plasmid

Catalog Number: 2-1323-000

Applications: Targeted Protein Expression

Type: Expression Vector

Species Reactivity: E. Coli

Size	List Price	Price	Cart
5 ug		$282.00	Add to Cart

Description	Expression plasmid. The expression cassette is under transcriptional control of the tetracycline promoter/operator. The expressed recombinant protein will be secreted into the periplasm
Affinity tag	Strep-Tactin ® affinity tag (Strep-tag II ®) for the purification of recombinant protein. The affinity tag is fused to the N-terminus of the recombinant protein.
Secretion	The ompA signal sequence directs the expressed protein into the periplasmic space and will be cleaved off during the translocation process.
Bacterial Expression	Expression is induced upon addition of 200 ug anhydrotetracycline (order no.: 2 0401 001; 2 0401 002) per 1 liter E. coli shaking culture (A₅₅₀= 0.5).
Expression strain	Any E. coli strain. The tet promoter works independently from the genetic background of E. coli.
Resistance	Chloramphenicol.Note: The CamR resistance gene codes for homotetrameric chloramphenicol acetyltransferase (MW of the monomer = 26.6 kDa) which is predominantly expressed in the cytosol of E.colitransformed with this plasmid
Form	5 ug, dissolved in 10 mM Tris/HCl pH 8.0, 1 mM EDTA; 20 ul.
Concentration	250 ng/ul.
Storage	4 °C for frequent usage, 20 °C for long term storage.

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