Strep-Tactin® Superflow® high capacity column, 5 x 0.2 ml

Product Details

Catalog Number: 2-1209-550

Storage: 2- 8 °C

Shipping: Room Temperature

Downloads: Data Sheet (pdf)

Downloads: MSDS (pdf)

Downloads: Protocol (pdf)

Downloads: Manual (pdf)

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Size	List Price	Price	Cart

As of 10/31/2024, Strep-Tactin® Sepharose® products have been discontinued. The replacement products are Strep-Tactin® 4Flow® High Capacity Products.

Replacement:

Name	Catalog Number
Strep-Tactin® 4Flow® High Capacity Mini-Column	2-1251-005

Advantages of Strep-Tactin® 4Flow® High Capacity vs. Strep-Tactin® Superflow®:

Matrix	Strep-Tactin® Superflow®	Strep-Tactin® 4Flow®
Bead structure	6% agarose, crosslinked	4% agarose, highly crosslinked
Bead size	60-160 um	50-150 um, spherical
Exclusion limit	6 x 10^6 Da	3 x 10^7 Da
Recommended flow rate	0.5-1 ml/min	0.5-1 ml/min
pH range for protein binding	7-8	7-8
Max pressue	9.6 bar	3.5 bar
Eluent	desthiobiotin	desthiobiotin
Regeneration buffer	Buffer R	100 mM NaOH

Gravity flowStrep-Tactin® Superflow® high capacity column for one-step purification of recombinant Strep-tag® proteins.

The Strep-Tactin® Superflow® high capacity resin provides 2 to 3 times higher binding capacity for Strep-tag® proteins than standard Strep-Tactin® Superflow®, and therefore, higher yields of highly pure Strep-tag® protein.

Dynamic binding capacity: 7mg protein/ml resin, determined with mCherry-Twin-Strep-tag® (30kDa).

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Product Publications

Jörg Scholl, et al. (2020). Phosphoenolpyruvate Carboxylase from the Cyanobacterium Synechocystis sp. PCC 6803 is Under Global Metabolic Control by PII Signaling. Molecular Microbiology, 114(2), 292-307. doi: 10.1111/mmi.14512
Fabian Mohr, et al. (2018). Efficient Immunoaffinity Chromatography of Lymphocytes Directly from Whole Blood. Scientific Reports, 8: 16731. doi: 10.1038/s41598-018-34589-z
Annegret Becker, et al. (2018). Functionalization of Gold-Nanoparticles by the Clostridium perfringens Enterotoxin C-Terminus for Tumor Cell Ablation Using the Gold Nanoparticle-Mediated Laser Perforation Technique. Scientific Reports, 8, 14963. doi: 10.1038/s41598-018-33392-0
Fabian Mohr, et al. (2017). Minimally Manipulated Murine Regulatory T Cells Purified by Reversible Fab Multimers are Potent Suppressors for Adoptive T-Cell Therapy. European Journal of Immunology, 47(12), 2153-2162. doi: 10.1002/eji.201747137
Ghérici Hassaine, et al. (2013). Large Scale Expression and Purification of the Mouse 5-HT3 Receptor. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1828(11), 2544-2552. doi: 10.1016/j.bbamem.2013.05.028
Christian Stemberger, et al. (2012). Novel Serial Positive Enrichment Technology Enables Clinical Multiparameter Cell Sorting. PLoS ONE, 7(4): e35798. doi: 10.1371/journal.pone.0035798

If you've used this product in a publication, let us know. Email pshuster@neuromics.com, with the publication details and you could be eligible for an Amazon gift card.

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