Gravity flow Strep-Tactin® Superflow® high capacity column

Product Details

Catalog Number: 2-1209-051

Applications: Protein Purification, FPLC

Type: Column

Product Sizes

Size	List Price	Price	Cart

As of 10/31/2024, Strep-Tactin® Sepharose® products have been discontinued. The replacement products are Strep-Tactin® 4Flow® High Capacity Products.

Replacement:

Name	Catalog Number
Strep-Tactin® 4Flow® High Capacity Column	2-1253-001

Advantages of Strep-Tactin® 4Flow® High Capacity vs. Strep-Tactin® Superflow®:

Matrix	Strep-Tactin® Superflow®	Strep-Tactin® 4Flow®
Bead structure	6% agarose, crosslinked	4% agarose, highly crosslinked
Bead size	60-160 um	50-150 um, spherical
Exclusion limit	6 x 10^6 Da	3 x 10^7 Da
Recommended flow rate	0.5-1 ml/min	0.5-1 ml/min
pH range for protein binding	7-8	7-8
Max pressue	9.6 bar	3.5 bar
Eluent	desthiobiotin	desthiobiotin
Regeneration buffer	Buffer R	100 mM NaOH

For purification of Strep-tag^® II proteins by gravity flow, low pressure or FPLC.

Strep-tag^®- Buffers and Reagents - Lysis, Elution, Regeneration, Blocking and Washing Buffers

Product Reviews
- - Rating:
LEAVE A REVIEW
Leave a review for this product and you could be eligible for a $10 Starbucks gift card.

Product Publications

Jörg Scholl, et al. (2020). Phosphoenolpyruvate Carboxylase from the Cyanobacterium Synechocystis sp. PCC 6803 is Under Global Metabolic Control by PII Signaling. Molecular Microbiology, 114(2), 292-307. doi: 10.1111/mmi.14512
Fabian Mohr, et al. (2018). Efficient Immunoaffinity Chromatography of Lymphocytes Directly from Whole Blood. Scientific Reports, 8: 16731. doi: 10.1038/s41598-018-34589-z
Annegret Becker, et al. (2018). Functionalization of Gold-Nanoparticles by the Clostridium perfringens Enterotoxin C-Terminus for Tumor Cell Ablation Using the Gold Nanoparticle-Mediated Laser Perforation Technique. Scientific Reports, 8, 14963. doi: 10.1038/s41598-018-33392-0
Fabian Mohr, et al. (2017). Minimally Manipulated Murine Regulatory T Cells Purified by Reversible Fab Multimers are Potent Suppressors for Adoptive T-Cell Therapy. European Journal of Immunology, 47(12), 2153-2162. doi: 10.1002/eji.201747137
Ghérici Hassaine, et al. (2013). Large Scale Expression and Purification of the Mouse 5-HT3 Receptor. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1828(11), 2544-2552. doi: 10.1016/j.bbamem.2013.05.028
Christian Stemberger, et al. (2012). Novel Serial Positive Enrichment Technology Enables Clinical Multiparameter Cell Sorting. PLoS ONE, 7(4): e35798. doi: 10.1371/journal.pone.0035798

If you've used this product in a publication, let us know. Email pshuster@neuromics.com, with the publication details and you could be eligible for an Amazon gift card.

Related Products

Name	Catalog #	Size
10x Buffer BXT; Strep-Tactin®XT Elution Buffer	2-1042-025	25 ml
Avidin	2-0204-050	50 mg
BioLock Biotin blocking solution	2-0205-050	50 ml
BioLock Biotin blocking solution	2-0205-250	250 ml
Buffer XT-R	2-1045-250	250ml
D-Biotin	2-1016-002	2 g
D-Biotin	2-1016-005	10 x 5 g
D-Biotin	2-1016-005	5 g
Desthiobiotin	2-1000-002	1 g
Desthiobiotin	2-1000-005	5 x 5 g
Desthiobiotin	2-1000-005	5 g
Empty Spin Columns	2-5150-050	50 Columns
GFP-Strep-tag control protein	2-1006-005	500 ug
GFP-Twin-Strep-tag control protein	2-1007-105	500 ul
Magnetic Separator	2-1602-000	1 Piece
MagStrep "type3" Strep-Tactin® beads	2-1613-002	2 ml
MagStrep "type3" XT beads 5% suspension	2-4090-010	10 ml
MagStrep "type3" XT beads 5% suspension	2-4090-010	10 x 10 ml
MagStrep Strep-Tactin®XT Beads	2-5090-002	2 ml
MagStrep Strep-Tactin®XT Beads	2-5090-010	10 x 10 ml
MagStrep Strep-Tactin®XT Beads	2-5090-010	10 ml
Strep-Tactin® 4Flow® High Capacity Column	2-1251-005	5 x 0.2 ml
Strep-Tactin® 4Flow® High Capacity Column	2-1252-001	5 x 1 ml
Strep-Tactin® 4Flow® High Capacity Column	2-1252-001	1 ml
Strep-Tactin® 4Flow® High Capacity Column	2-1253-001	5 ml
Strep-Tactin® 4Flow® High Capacity Column	2-1253-001	5 x 5 ml
Strep-Tactin® 4Flow® High Capacity FPLC Column	2-1257-001	1 ml
Strep-Tactin® 4Flow® High Capacity FPLC Column	2-1257-001	10 x 1 ml
Strep-Tactin® 4Flow® High Capacity FPLC Column	2-1258-001	5 ml
Strep-Tactin® 4Flow® High Capacity FPLC Column	2-1258-001	10 x 5 ml
Strep-Tactin® 4Flow® High Capacity Resin	2-1250-002	4 ml
Strep-Tactin® 4Flow® High Capacity Resin	2-1250-010	20 ml
Strep-Tactin® 4Flow® High Capacity Resin	2-1250-010	5 x 20 ml
Strep-Tactin® 4Flow® High Capacity Resin	2-1250-025	5 x 50 ml
Strep-Tactin® 4Flow® High Capacity Resin	2-1250-025	50 ml
Strep-Tactin® 4Flow® High Capacity Resin	2-1250-025	10 x 50 ml
Strep-Tactin® MacroPrep® resin	2-1505-010	20 ml
Strep-Tactin® MacroPrep® resin	2-1505-010	5 x 20 ml
Strep-Tactin®XT 4Flow® cartridge	2-5023-001	10 x 1 ml
Strep-Tactin®XT 4Flow® cartridge	2-5023-001	1 ml
Strep-Tactin®XT 4Flow® cartridge	2-5024-001	5 ml
Strep-Tactin®XT 4Flow® cartridge	2-5024-001	10 x 5 ml
Strep-Tactin®XT 4Flow® column	2-5011-005	5 x 0.2 ml
Strep-Tactin®XT 4Flow® column	2-5012-001	5 x 1 ml
Strep-Tactin®XT 4Flow® column	2-5012-001	1 ml
Strep-Tactin®XT 4Flow® column	2-5013-001	5 x 5 ml
Strep-Tactin®XT 4Flow® column	2-5013-001	5 ml
Strep-Tactin®XT 4Flow® column	2-5014-001	10 ml
Strep-Tactin®XT 4Flow® high capacity cartridge	2-5027-001	10 x 1 ml
Strep-Tactin®XT 4Flow® high capacity cartridge	2-5027-001	1 ml
Strep-Tactin®XT 4Flow® high capacity cartridge	2-5028-001	10 x 5 ml
Strep-Tactin®XT 4Flow® high capacity cartridge	2-5028-001	5 ml
Strep-Tactin®XT 4Flow® high capacity column	2-5031-005	5 x 0.2 ml
Strep-Tactin®XT 4Flow® high capacity column	2-5032-001	5 x 1 ml
Strep-Tactin®XT 4Flow® high capacity column	2-5032-001	1 ml
Strep-Tactin®XT 4Flow® high capacity column	2-5033-001	5 ml
Strep-Tactin®XT 4Flow® high capacity column	2-5033-001	5 x 5 ml
Strep-Tactin®XT 4Flow® high capacity column	2-5034-001	10 ml
Strep-Tactin®XT 4Flow® high capacity resin	2-5030-002	4 ml
Strep-Tactin®XT 4Flow® high capacity resin	2-5030-010	5 x 20 ml
Strep-Tactin®XT 4Flow® high capacity resin	2-5030-010	20 ml
Strep-Tactin®XT 4Flow® high capacity resin	2-5030-025	10 x 50 ml
Strep-Tactin®XT 4Flow® high capacity resin	2-5030-025	50 ml
Strep-Tactin®XT 4Flow® high capacity resin	2-5030-025	5 x 50 ml
Strep-Tactin®XT 4Flow® high capacity Spin Column Kit	2-5151-000	1 Kit
Strep-Tactin®XT 4Flow® resin	2-5010-002	4 ml
Strep-Tactin®XT 4Flow® resin	2-5010-010	5 x 20 ml
Strep-Tactin®XT 4Flow® resin	2-5010-010	20 ml
Strep-Tactin®XT 4Flow® resin	2-5010-025	50 ml
Strep-Tactin®XT 4Flow® resin	2-5010-025	5 x 50 ml
Strep-Tactin®XT 4Flow® resin	2-5010-025	10 x 10 ml
Strep-Tactin®XT 4Flow® Starter Kit	2-5998-000	1 kit
Strep-tag II Desthiobiotin elution reagent	2-1000-025	25 ml
Strep-tag II Peptide	2-1018-002	5 x 1.8 mg
Strep-tag II Peptide	2-1018-002	1.8 mg
Strep-tag regeneration buffer with HABA	2-1002-100	100 ml
Strep-tag washing buffer	2-1003-100	100 ml
WET FRED For 1 ml Gravity flow columns	2-0911-001	1 Set
WET FRED For 5-10 ml Gravity flow columns	2-0910-001	1 Set

Products

Customer Publications

Ordering Info & Policy

Blog Posts

Strep-Tactin® Superflow-5 ml

Product Details

Product Sizes

Product Publications

Related Products

Images