Strep-Tactin® Superflow® high capacity 50% suspension

Product Details

Catalog Number: 2-1208-025

Product Sizes

Size	List Price	Price	Cart

As of 10/31/2024, Strep-Tactin® Sepharose® products have been discontinued. The replacement products are Strep-Tactin® 4Flow® High Capacity Products.

Replacement:

Name	Catalog Number
Strep-Tactin® 4Flow® High Capacity Resin	2-1250-025

Advantages of Strep-Tactin® 4Flow® High Capacity vs. Strep-Tactin® Superflow®:

Matrix	Strep-Tactin® Superflow®	Strep-Tactin® 4Flow®
Bead structure	6% agarose, crosslinked	4% agarose, highly crosslinked
Bead size	60-160 um	50-150 um, spherical
Exclusion limit	6 x 10^6 Da	3 x 10^7 Da
Recommended flow rate	0.5-1 ml/min	0.5-1 ml/min
pH range for protein binding	7-8	7-8
Max pressue	9.6 bar	3.5 bar
Eluent	desthiobiotin	desthiobiotin
Regeneration buffer	Buffer R	100 mM NaOH

The Strep-Tactin® Superflow® high capacity resin provides 2 to 3 times higher binding capacity for strep-tag proteins than standard Strep-Tactin® Superflow® and therefore, higher yields of highly pure Strep-tag® protein.

Strep-Tactin® Superflow® high capacity is used for purification of recombinant Strep-tag® proteins under gravity flow and low pressure or FPLC . Bulk resin is recommended for packing of our columns and cartridges since the resin is optimized for column affinity chromatography rather than for batch purification.

Binding capacity: 1 ml sedimented resin (corresponding to 2 ml of a 50 % suspension) is useful for the one-step purification of 150 to 500 nmol or 3 -15 mg recombinant protein.

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Product Publications

Kerstin Wernike, et al. (2021). Differentiation of Antibodies against Selected Simbu Serogroup Viruses by a Glycoprotein Gc-Based Triplex ELISA. Veterinary Sciences, 8(1), 12. doi: 10.3390/vetsci8010012
Gleyder Roman-Sosa, et al. (2016). Analysis of the Humoral Immune Response Against the Envelope Glycoprotein Gc of Schmallenberg Virus Reveals a Domain Located At the Amino Terminus Targeted by mAbs with Neutralizing Activity. Journal of General Virology, 97(3). doi: 10.1099/jgv.0.000377

If you've used this product in a publication, let us know. Email pshuster@neuromics.com, with the publication details and you could be eligible for an Amazon gift card.

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