Strep-Tactin® Superflow® high capacity 50% suspension

Product Details

Catalog Number: 2-1208-025

Product Sizes

Size	List Price	Price	Cart

As of 10/31/2024, Strep-Tactin® Sepharose® products have been discontinued. The replacement products are Strep-Tactin® 4Flow® High Capacity Products.

Replacement:

Name	Catalog Number
Strep-Tactin® 4Flow® High Capacity Resin	2-1250-025

Advantages of Strep-Tactin® 4Flow® High Capacity vs. Strep-Tactin® Superflow®:

Matrix	Strep-Tactin® Superflow®	Strep-Tactin® 4Flow®
Bead structure	6% agarose, crosslinked	4% agarose, highly crosslinked
Bead size	60-160 um	50-150 um, spherical
Exclusion limit	6 x 10^6 Da	3 x 10^7 Da
Recommended flow rate	0.5-1 ml/min	0.5-1 ml/min
pH range for protein binding	7-8	7-8
Max pressue	9.6 bar	3.5 bar
Eluent	desthiobiotin	desthiobiotin
Regeneration buffer	Buffer R	100 mM NaOH

The Strep-Tactin® Superflow® high capacity resin provides 2 to 3 times higher binding capacity for strep-tag proteins than standard Strep-Tactin® Superflow® and therefore, higher yields of highly pure Strep-tag® protein.

Strep-Tactin® Superflow® high capacity is used for purification of recombinant Strep-tag® proteins under gravity flow and low pressure or FPLC . Bulk resin is recommended for packing of our columns and cartridges since the resin is optimized for column affinity chromatography rather than for batch purification.

Binding capacity: 1 ml sedimented resin (corresponding to 2 ml of a 50 % suspension) is useful for the one-step purification of 150 to 500 nmol or 3 -15 mg recombinant protein.

Product Reviews

Product Publications

Kerstin Wernike, et al. (2021). Differentiation of Antibodies against Selected Simbu Serogroup Viruses by a Glycoprotein Gc-Based Triplex ELISA. Veterinary Sciences, 8(1), 12. doi: 10.3390/vetsci8010012
Gleyder Roman-Sosa, et al. (2016). Analysis of the Humoral Immune Response Against the Envelope Glycoprotein Gc of Schmallenberg Virus Reveals a Domain Located At the Amino Terminus Targeted by mAbs with Neutralizing Activity. Journal of General Virology, 97(3). doi: 10.1099/jgv.0.000377

If you've used this product in a publication, let us know. Email pshuster@neuromics.com, with the publication details and you could be eligible for an Amazon gift card.

Related Products

Name	Catalog #	Size
10x Buffer BXT; Strep-Tactin®XT Elution Buffer	2-1042-025	25 ml
Avidin	2-0204-050	50 mg
BioLock Biotin blocking solution	2-0205-050	50 ml
BioLock Biotin blocking solution	2-0205-250	250 ml
Biotin	2-1016-002	2 g
Biotin	2-1016-005	5 g
Buffer XT-R	2-1045-250	250ml
Desthiobiotin	2-1000-002	1 g
Desthiobiotin	2-1000-005	5 g
Empty Spin Columns	2-5150-050	50 Columns
GFP-Strep-tag control protein	2-1006-005	500 ug
GFP-Twin-Strep-tag control protein	2-1007-105	500 ul
Magnetic Separator	2-1602-000	1 Piece
MagStrep "type3" Strep-Tactin® beads	2-1613-002	2 ml
MagStrep Strep-Tactin®XT Beads	2-5090-002	2 ml
MagStrep Strep-Tactin®XT Beads	2-5090-010	10 ml
Strep-Tactin® 4Flow® High Capacity Column	2-1251-005	5 x 0.2 ml
Strep-Tactin® 4Flow® High Capacity Column	2-1252-001	1 ml
Strep-Tactin® 4Flow® High Capacity Column	2-1253-001	5 ml
Strep-Tactin® 4Flow® High Capacity FPLC Column	2-1257-001	1 ml
Strep-Tactin® 4Flow® High Capacity FPLC Column	2-1257-001	10 x 1 ml
Strep-Tactin® 4Flow® High Capacity FPLC Column	2-1258-001	5 ml
Strep-Tactin® 4Flow® High Capacity FPLC Column	2-1258-001	10 x 5 ml
Strep-Tactin® 4Flow® High Capacity Resin	2-1250-002	4 ml
Strep-Tactin® 4Flow® High Capacity Resin	2-1250-010	20 ml
Strep-Tactin® 4Flow® High Capacity Resin	2-1250-025	50 ml
Strep-Tactin®XT 4Flow® cartridge	2-5023-001	1 ml
Strep-Tactin®XT 4Flow® cartridge	2-5023-001	10 x 1 ml
Strep-Tactin®XT 4Flow® cartridge	2-5024-001	10 x 5 ml
Strep-Tactin®XT 4Flow® cartridge	2-5024-001	5 ml
Strep-Tactin®XT 4Flow® column	2-5011-005	5 x 0.2 ml
Strep-Tactin®XT 4Flow® column	2-5012-001	1 ml
Strep-Tactin®XT 4Flow® column	2-5013-001	5 ml
Strep-Tactin®XT 4Flow® column	2-5014-001	10 ml
Strep-Tactin®XT 4Flow® high capacity cartridge	2-5027-001	1 ml
Strep-Tactin®XT 4Flow® high capacity cartridge	2-5027-001	10 x 1 ml
Strep-Tactin®XT 4Flow® high capacity cartridge	2-5028-001	5 ml
Strep-Tactin®XT 4Flow® high capacity cartridge	2-5028-001	10 x 5 ml
Strep-Tactin®XT 4Flow® high capacity column	2-5031-005	5 x 0.2 ml
Strep-Tactin®XT 4Flow® high capacity column	2-5032-001	1 ml
Strep-Tactin®XT 4Flow® high capacity column	2-5033-001	5 ml
Strep-Tactin®XT 4Flow® high capacity column	2-5034-001	10 ml
Strep-Tactin®XT 4Flow® high capacity resin	2-5030-002	4 ml
Strep-Tactin®XT 4Flow® high capacity resin	2-5030-010	20 ml
Strep-Tactin®XT 4Flow® high capacity resin	2-5030-025	50 ml
Strep-Tactin®XT 4Flow® high capacity resin	2-5030-025	5 x 50 ml
Strep-Tactin®XT 4Flow® high capacity Spin Column Kit	2-5151-000	1 Kit
Strep-Tactin®XT 4Flow® resin	2-5010-002	4 ml
Strep-Tactin®XT 4Flow® resin	2-5010-010	20 ml
Strep-Tactin®XT 4Flow® resin	2-5010-025	50 ml
Strep-Tactin®XT 4Flow® Starter Kit	2-5998-000	1 kit
Strep-tag II Desthiobiotin elution reagent	2-1000-025	25 ml
Strep-tag II Peptide	2-1018-002	1.8 mg
Strep-tag regeneration buffer with HABA	2-1002-100	100 ml
Strep-tag washing buffer	2-1003-100	100 ml
WET FRED For 1 ml Gravity flow columns	2-0911-001	1 Set
WET FRED For 5-10 ml Gravity flow columns	2-0910-001	1 Set

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