Gravity flow Strep-Tactin® Sepharose® column

Product Details

Catalog Number: 2-1202-001

Applications: Protein Purification

Type: Column

Product Sizes

Size	List Price	Price	Cart
1 ml		$112.00	Add to Cart
5 x 1 ml		$495.00	Add to Cart

On 10/31/2024, Strep-Tactin® Sepharose® products will be discontinued and replaced with the new and improved Strep-Tactin® 4Flow® High Capacity Products.

Replacement:

Name	Catalog Number
Strep-Tactin® 4Flow® High Capacity Column	2-1250-025

Advantages of Strep-Tactin® 4Flow® High Capacity vs. Strep-Tactin® Sepharose®:

Matrix	Strep-Tactin® Sepharose®	Strep-Tactin® 4Flow®
Bead structure	4% agarose, crosslinked	4% agarose, highly crosslinked
Bead size	45-165 um	50-150 um, spherical
Exclusion limit	~3 x 10^7 Da	3 x 10^7 Da
Recommended flow rate	gravity flow	0.5-1 ml/min
pH range for protein binding	7-8	7-8
Max pressue	gravity flow	3.5 bar
Eluent	desthiobiotin	desthiobiotin
Regeneration buffer	Buffer R	100 mM NaOH

Product Reviews
LEAVE A REVIEW
Leave a review for this product and you could be eligible for a $10 Starbucks gift card.

Product Publications

Laura Prioretti, et al. (2023). Carbon Fixation in the Chemolithoautotrophic Bacterium Aquifex aeolicus Involves Two Low-Potential Ferredoxins as Partners of the PFOR and OGOR Enzymes. Life, 13(3), 627. doi: 10.3390/life13030627
Yun Nan Hou, et al. (2022). A Conformation-Specific Nanobody Targeting the Nicotinamide Mononucleotide-Activated State of SARM1. Nature Communications, 13, 7898. doi: 10.1038/s41467-022-35581-y
Lukas Muschallik, et al. (2020). Synthesis of α-hydroxy Ketones and Vicinal (R,R)-Diols by Bacillus clausii DSM 8716T Butanediol Dehydrogenase. RSC Advances, 10, 12206-12216. doi: 10.1039/D0RA02066D
Christina S. Heil, et al. (2018). Site-Specific Labeling of Multidomain Proteins by Amber Codon Suppression. Scientific Reports, 8, 14864. doi: 10.1038/s41598-018-33115-5
Christophe J. Lalaurie, et al. (2018). The de novo Design of a Biocompatible and Functional Integral Membrane Protein Using Minimal Sequence Complexity. Scientific Reports, 8, 14564. doi: 10.1038/s41598-018-31964-8
Nicole Schäfer, et al. (2016). Complement Regulator FHR-3 Is Elevated either Locally or Systemically in a Selection of Autoimmune Diseases. Frontiers in Immunology, 7, doi: 10.3389/fimmu.2016.00542
Cyril Barinka, et al. (2016). Selection and Characterization of Anticalins Targeting Human Prostate-Specific Membrane Antigen (PSMA). Protein Engineering, Design and Selection, 29(3), 105–115. doi: 10.1093/protein/gzv065
Sebastian Dintner, et al. (2014). A Sensory Complex Consisting of an ATP-binding Cassette Transporter and a Two-component Regulatory System Controls Bacitracin Resistance in Bacillus subtilis. Journal of Biological Chemistry, 289(40), 27899-27910. doi: 10.1074/jbc.M114.596221

If you've used this product in a publication, let us know. Email pshuster@neuromics.com, with the publication details and you could be eligible for an Amazon gift card.

Related Products

Name	Catalog #	Size
10x Buffer BXT; Strep-Tactin®XT Elution Buffer	2-1042-025	25 ml
Avidin	2-0204-050	50 mg
BioLock Biotin blocking solution	2-0205-050	50 ml
BioLock Biotin blocking solution	2-0205-250	250 ml
Buffer XT-R	2-1045-250	250ml
D-Biotin	2-1016-002	2 g
D-Biotin	2-1016-005	10 x 5 g
D-Biotin	2-1016-005	5 g
Desthiobiotin	2-1000-002	1 g
Desthiobiotin	2-1000-005	5 g
Desthiobiotin	2-1000-005	5 x 5 g
Empty Spin Columns	2-5150-050	50 Columns
GFP-Strep-tag control protein	2-1006-005	500 ug
GFP-Twin-Strep-tag control protein	2-1007-105	500 ul
Magnetic Separator	2-1602-000	1 Piece
MagStrep "type3" Strep-Tactin® beads	2-1613-002	2 ml
MagStrep "type3" XT beads 5% suspension	2-4090-010	10 ml
MagStrep "type3" XT beads 5% suspension	2-4090-010	10 x 10 ml
MagStrep Strep-Tactin®XT Beads	2-5090-002	2 ml
MagStrep Strep-Tactin®XT Beads	2-5090-010	10 ml
MagStrep Strep-Tactin®XT Beads	2-5090-010	10 x 10 ml
Strep-Tactin Sepharose 50% suspension	2-1201-002	4 ml
Strep-Tactin Sepharose 50% suspension	2-1201-010	20 ml
Strep-Tactin Sepharose 50% suspension	2-1201-010	5 x 20 ml
Strep-Tactin Sepharose 50% suspension	2-1201-025	10 x 50 ml
Strep-Tactin Sepharose 50% suspension	2-1201-025	5 x 50 ml
Strep-Tactin Sepharose 50% suspension	2-1201-025	50 ml
Strep-Tactin Superflow 50% suspension	2-1206-025	10 x 50 ml
Strep-Tactin Superflow 50% suspension	2-1206-025	5 x 50 ml
Strep-Tactin Superflow 50% suspension	2-1206-025	50 ml
Strep-Tactin® 4Flow® High Capacity Column	2-1251-005	5 x 0.2 ml
Strep-Tactin® 4Flow® High Capacity Column	2-1252-001	1 ml
Strep-Tactin® 4Flow® High Capacity Column	2-1252-001	5 x 1 ml
Strep-Tactin® 4Flow® High Capacity Column	2-1253-001	5 ml
Strep-Tactin® 4Flow® High Capacity Column	2-1253-001	5 x 5 ml
Strep-Tactin® 4Flow® High Capacity FPLC Column	2-1257-001	1 ml
Strep-Tactin® 4Flow® High Capacity FPLC Column	2-1257-001	10 x 1 ml
Strep-Tactin® 4Flow® High Capacity FPLC Column	2-1258-001	10 x 5 ml
Strep-Tactin® 4Flow® High Capacity FPLC Column	2-1258-001	5 ml
Strep-Tactin® 4Flow® High Capacity Resin	2-1250-002	4 ml
Strep-Tactin® 4Flow® High Capacity Resin	2-1250-010	20 ml
Strep-Tactin® 4Flow® High Capacity Resin	2-1250-010	5 x 20 ml
Strep-Tactin® 4Flow® High Capacity Resin	2-1250-025	10 x 50 ml
Strep-Tactin® 4Flow® High Capacity Resin	2-1250-025	5 x 50 ml
Strep-Tactin® 4Flow® High Capacity Resin	2-1250-025	50 ml
Strep-Tactin® MacroPrep® resin	2-1505-010	20 ml
Strep-Tactin® MacroPrep® resin	2-1505-010	5 x 20 ml
Strep-Tactin® Superflow® high capacity 50% suspension	2-1208-002	4 ml
Strep-Tactin® Superflow® high capacity 50% suspension	2-1208-010	20 ml
Strep-Tactin® Superflow® high capacity 50% suspension	2-1208-010	5 x 20 ml
Strep-Tactin® Superflow® high capacity 50% suspension	2-1208-025	10 x 50 ml
Strep-Tactin® Superflow® high capacity 50% suspension	2-1208-025	5 x 50 ml
Strep-Tactin® Superflow® high capacity 50% suspension	2-1208-025	50 ml
Strep-Tactin® Superflow® high capacity cartridge	2-1239-001	1 ml
Strep-Tactin® Superflow® high capacity cartridge	2-1239-001	10 x 1 ml
Strep-Tactin® Superflow® high capacity cartridge	2-1240-001	10 x 5 ml
Strep-Tactin® Superflow® high capacity cartridge	2-1240-001	5 ml
Strep-Tactin® Superflow® high capacity cartridge	2-1240-001	5 x 5 ml
Strep-Tactin® Superflow® high capacity column	2-1209-550	5 x 0.2 ml
Strep-Tactin® Superflow-1X1 ml	2-1209-001	1 ml
Strep-Tactin® Superflow-1X1 ml	2-1209-001	10 x 1 ml
Strep-Tactin® Superflow-1X1 ml	2-1209-001	5 x 1 ml
Strep-Tactin® Superflow-5 ml	2-1209-051	5 ml
Strep-Tactin® Superflow-5 ml	2-1209-051	5 x 5 ml
Strep-Tactin®XT 4Flow® cartridge	2-5023-001	1 ml
Strep-Tactin®XT 4Flow® cartridge	2-5023-001	10 x 1 ml
Strep-Tactin®XT 4Flow® cartridge	2-5024-001	10 x 5 ml
Strep-Tactin®XT 4Flow® cartridge	2-5024-001	5 ml
Strep-Tactin®XT 4Flow® column	2-5011-005	5 x 0.2 ml
Strep-Tactin®XT 4Flow® column	2-5012-001	1 ml
Strep-Tactin®XT 4Flow® column	2-5012-001	5 x 1 ml
Strep-Tactin®XT 4Flow® column	2-5013-001	5 ml
Strep-Tactin®XT 4Flow® column	2-5013-001	5 x 5 ml
Strep-Tactin®XT 4Flow® column	2-5014-001	10 ml
Strep-Tactin®XT 4Flow® high capacity cartridge	2-5027-001	1 ml
Strep-Tactin®XT 4Flow® high capacity cartridge	2-5027-001	10 x 1 ml
Strep-Tactin®XT 4Flow® high capacity cartridge	2-5028-001	10 x 5 ml
Strep-Tactin®XT 4Flow® high capacity cartridge	2-5028-001	5 ml
Strep-Tactin®XT 4Flow® high capacity column	2-5031-005	5 x 0.2 ml
Strep-Tactin®XT 4Flow® high capacity column	2-5032-001	1 ml
Strep-Tactin®XT 4Flow® high capacity column	2-5032-001	5 x 1 ml
Strep-Tactin®XT 4Flow® high capacity column	2-5033-001	5 ml
Strep-Tactin®XT 4Flow® high capacity column	2-5033-001	5 x 5 ml
Strep-Tactin®XT 4Flow® high capacity column	2-5034-001	10 ml
Strep-Tactin®XT 4Flow® high capacity resin	2-5030-002	4 ml
Strep-Tactin®XT 4Flow® high capacity resin	2-5030-010	20 ml
Strep-Tactin®XT 4Flow® high capacity resin	2-5030-010	5 x 20 ml
Strep-Tactin®XT 4Flow® high capacity resin	2-5030-025	10 x 50 ml
Strep-Tactin®XT 4Flow® high capacity resin	2-5030-025	5 x 50 ml
Strep-Tactin®XT 4Flow® high capacity resin	2-5030-025	50 ml
Strep-Tactin®XT 4Flow® high capacity Spin Column Kit	2-5151-000	1 Kit
Strep-Tactin®XT 4Flow® resin	2-5010-002	4 ml
Strep-Tactin®XT 4Flow® resin	2-5010-010	20 ml
Strep-Tactin®XT 4Flow® resin	2-5010-010	5 x 20 ml
Strep-Tactin®XT 4Flow® resin	2-5010-025	10 x 10 ml
Strep-Tactin®XT 4Flow® resin	2-5010-025	5 x 50 ml
Strep-Tactin®XT 4Flow® resin	2-5010-025	50 ml
Strep-Tactin®XT 4Flow® Starter Kit	2-5998-000	1 kit
Strep-Tactin®XT Buffer Set	2-1043-000	1 Set
Strep-tag II Desthiobiotin elution reagent	2-1000-025	25 ml
Strep-tag II Peptide	2-1018-002	1.8 mg
Strep-tag II Peptide	2-1018-002	5 x 1.8 mg
Strep-tag protein purification buffer set	2-1002-001	1 Buffer Set
Strep-tag regeneration buffer with HABA	2-1002-100	100 ml
Strep-tag washing buffer	2-1003-100	100 ml
WET FRED For 1 ml Gravity flow columns	2-0911-001	1 Set
WET FRED For 5-10 ml Gravity flow columns	2-0910-001	1 Set

Images

A 36 kDa enzyme and a mutant, both with C-terminal Strep-tag II, were expressed in the cytoplasm of E. coli. 1: Sample of crude lysate after cytosolic expression with pASK-IBA3 2: Sample of flow through during chromatography 3: Sample after the addition of 2.5 mM desthiobiotin M: Molecular size standard (kDa)

Products

Customer Publications

Ordering Info & Policy

Blog Posts

Strep-Tactin® Sepharose Gravityflow-1 ml

Product Details

Product Sizes

Product Publications

Related Products

Images