Strep-Tactin® Sepharose® 50% suspension

Product Details

Catalog Number: 2-1201-010

Applications: Protein Purification

Type: Column Suspensions

Product Sizes

Size	List Price	Price	Cart

As of 10/31/2024, Strep-Tactin® Sepharose® products have been discontinued. The replacement products are Strep-Tactin® 4Flow® High Capacity Products.

Replacement:

Name	Catalog Number
Strep-Tactin® 4Flow® High Capacity Resin	2-1250-010

Advantages of Strep-Tactin® 4Flow® High Capacity vs. Strep-Tactin® Sepharose®:

Matrix	Strep-Tactin® Sepharose®	Strep-Tactin® 4Flow®
Bead structure	4% agarose, crosslinked	4% agarose, highly crosslinked
Bead size	45-165 um	50-150 um, spherical
Exclusion limit	~3 x 10^7 Da	3 x 10^7 Da
Recommended flow rate	gravity flow	0.5-1 ml/min
pH range for protein binding	7-8	7-8
Max pressue	gravity flow	3.5 bar
Eluent	desthiobiotin	desthiobiotin
Regeneration buffer	Buffer R	100 mM NaOH

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Product Publications

Jinkun Xu, et al. (2022). Conformational Changes of a Phosphatidylcholine Flippase in Lipid Membranes. Cell Reports, 38(11), 110518. doi: 10.1016/j.celrep.2022.110518
Xiaonan Liu, et al. (2021). SARS-CoV-2–Host Proteome Interactions for Antiviral Drug Discovery. Molecular Systems Biology, 17(11), e10396. doi: 10.15252/msb.202110396
Kuo Zhang, et al. (2021). Structural Insights into Viral RNA Capping and Plasma Membrane Targeting by Chikungunya Virus Nonstructural Protein 1. Cell Host & Microbe, 29(5), 757-764. doi: 10.1016/j.chom.2021.02.018
Bing Rao, et al. (2021). The Cryo-EM Structure of an ERAD Protein Channel Formed by Tetrameric Human Derlin-1. Science Advances, 7(10). doi: 10.1126/sciadv.abe8591
Chunyan Wang, et al. (2020). A Human Monoclonal Antibody Blocking SARS-CoV-2 Infection. Nature Communications, 11, 2251. doi: 10.1038/s41467-020-16256-y
Yasunori Watanabe, et al. (2020). Site-Specific Glycan Analysis of the SARS-CoV-2 Spike. Science, 369(6501), 330-333. doi: 10.1126/science.abb9983
Jung Hwan Hwang, et al. (2019). Gadd45β Promotes Regeneration After Injury Through TGFβ-Dependent Restitution in Experimental Colitis. Experimental & Molecular Medicine, 51, 1–14. doi: 10.1038/s12276-019-0335-y
Jana Krieger, et al. (2018). Cationic Domains in Particle-Forming and Assembly-Deficient HBV Core Antigens Capture Mammalian RNA that stimulates Th1-Biased Antibody Responses by DNA Vaccination. Scientific Reports, 8, 14660. doi: 10.1038/s41598-018-32971-5
Francisco Romero Pastrana, et al. (2017). A Lactococcus lactis Expression Vector Set with Multiple Affinity Tags to Facilitate Isolation and Direct Labeling of Heterologous Secreted Proteins. Applied Microbiology and Biotechnology, 101, 8139–8149. doi: 10.1007/s00253-017-8524-x
Franziska Tausch, et al. (2017). Evidence for Complex Formation of the Bacillus cereus Haemolysin BL Components in Solution. Toxins, 9(9), 288. doi: 10.3390/toxins9090288
Markus Mörk-Mörkenstein, et al. (2017). Non-Canonical Activation of Histidine Kinase KdpD by Phosphotransferase Protein PtsN through Interaction with the Transmitter Domain. Molecular Microbiology, 106(1), 54-73. doi: 10.1111/mmi.13751
Oliver Pänke, et al. (2000). F-ATPase: Specific Observation of the Rotating C Subunit Oligomer of EFoEF1. FEBS Letters, 472(1), 34-38. doi: 10.1016/S0014-5793(00)01436-8

If you've used this product in a publication, let us know. Email pshuster@neuromics.com, with the publication details and you could be eligible for an Amazon gift card.

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Strep-Tactin Sepharose 50% suspension

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